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Distribution and function of the hydrogen sulfide-sensitive TRPA1 ion channel in rat urinary bladder.

Publication ,  Journal Article
Streng, T; Axelsson, HE; Hedlund, P; Andersson, DA; Jordt, S-E; Bevan, S; Andersson, K-E; Högestätt, ED; Zygmunt, PM
Published in: Eur Urol
February 2008

OBJECTIVES: To investigate the distribution of the transient receptor potential (TRP) A1 ion channel in the rat urinary bladder, and to study the effects of hydrogen sulfide (H(2)S) and known TRPA1 activators on micturition in conscious rats and on heterologously expressed ion channels. METHODS: The expression of TRPA1 in urinary bladder was studied with fluorescence immunohistochemistry and real-time PCR in female Sprague-Dawley rats. Cystometric investigations were performed in conscious animals subjected to intravesical administration of sodium hydrogen sulfide (NaHS, donor of H(2)S), allyl isothiocyanate (AI), and cinnamaldehyde (CA). Fluorometric calcium imaging was used to study the effect of NaHS on human and mouse TRPA1 expressed in CHO cells. RESULTS: TRPA1 immunoreactivity was found on unmyelinated nerve fibres within the urothelium, suburothelial space, and muscle layer as well as around blood vessels throughout the bladder. All TRPA1 immunoreactive nerves fibres also expressed TRPV1 immunoreactivity and vice versa. TRPA1 was also detected in urothelial cells at both transcriptional and protein levels. AI increased micturition frequency and reduced voiding volume. CA and NaHS produced similar changes in urodynamic parameters after disruption of the urothelial barrier with protamine sulfate. NaHS also induced calcium responses in TRPA1-expressing CHO cells, but not in untransfected cells. CONCLUSIONS: The expression of TRPA1 on C-fibre bladder afferents and urothelial cells together with the finding that intravesical TRPA1 activators initiate detrusor overactivity indicate that TRPA1 may have a role in sensory transduction in this organ. The study also highlights H(2)S as a TRPA1 activator potentially involved in inflammatory bladder disease.

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Published In

Eur Urol

DOI

ISSN

0302-2838

Publication Date

February 2008

Volume

53

Issue

2

Start / End Page

391 / 399

Location

Switzerland

Related Subject Headings

  • Urology & Nephrology
  • Urination
  • Urinary Bladder
  • TRPC Cation Channels
  • TRPA1 Cation Channel
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats, Sprague-Dawley
  • Rats
  • Neurons, Afferent
  • Nerve Fibers, Unmyelinated
 

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Streng, T., Axelsson, H. E., Hedlund, P., Andersson, D. A., Jordt, S.-E., Bevan, S., … Zygmunt, P. M. (2008). Distribution and function of the hydrogen sulfide-sensitive TRPA1 ion channel in rat urinary bladder. Eur Urol, 53(2), 391–399. https://doi.org/10.1016/j.eururo.2007.10.024
Streng, Tomi, Helena E. Axelsson, Petter Hedlund, David A. Andersson, Sven-Eric Jordt, Stuart Bevan, Karl-Erik Andersson, Edward D. Högestätt, and Peter M. Zygmunt. “Distribution and function of the hydrogen sulfide-sensitive TRPA1 ion channel in rat urinary bladder.Eur Urol 53, no. 2 (February 2008): 391–99. https://doi.org/10.1016/j.eururo.2007.10.024.
Streng T, Axelsson HE, Hedlund P, Andersson DA, Jordt S-E, Bevan S, et al. Distribution and function of the hydrogen sulfide-sensitive TRPA1 ion channel in rat urinary bladder. Eur Urol. 2008 Feb;53(2):391–9.
Streng, Tomi, et al. “Distribution and function of the hydrogen sulfide-sensitive TRPA1 ion channel in rat urinary bladder.Eur Urol, vol. 53, no. 2, Feb. 2008, pp. 391–99. Pubmed, doi:10.1016/j.eururo.2007.10.024.
Streng T, Axelsson HE, Hedlund P, Andersson DA, Jordt S-E, Bevan S, Andersson K-E, Högestätt ED, Zygmunt PM. Distribution and function of the hydrogen sulfide-sensitive TRPA1 ion channel in rat urinary bladder. Eur Urol. 2008 Feb;53(2):391–399.
Journal cover image

Published In

Eur Urol

DOI

ISSN

0302-2838

Publication Date

February 2008

Volume

53

Issue

2

Start / End Page

391 / 399

Location

Switzerland

Related Subject Headings

  • Urology & Nephrology
  • Urination
  • Urinary Bladder
  • TRPC Cation Channels
  • TRPA1 Cation Channel
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats, Sprague-Dawley
  • Rats
  • Neurons, Afferent
  • Nerve Fibers, Unmyelinated