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Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption.

Publication ,  Journal Article
Bösl, MR; Stein, V; Hübner, C; Zdebik, AA; Jordt, SE; Mukhopadhyay, AK; Davidoff, MS; Holstein, AF; Jentsch, TJ
Published in: EMBO J
March 15, 2001

The functions of some CLC Cl(-) channels are evident from human diseases that result from their mutations, but the role of the broadly expressed ClC-2 Cl(-) channel is less clear. Several important functions have been attributed to ClC-2, but contrary to these expectations ClC-2-deficient mice lacked overt abnormalities except for a severe degeneration of the retina and the testes, which led to selective male infertility. Seminiferous tubules did not develop lumina and germ cells failed to complete meiosis. Beginning around puberty there was a massive death of primary spermatocytes and later also of spermatogonia. Tubules were filled with abnormal Sertoli cells, which normally express ClC-2 in patches adjacent to germ cells. In the retina, photoreceptors lacked normal outer segments and degenerated between days P10 and P30. The current across the retinal pigment epithelium was severely reduced at P36. Thus, ClC-2 disruption entails the death of two cell types which depend on supporting cells that form the blood-testes and blood-retina barriers. We propose that ClC-2 is crucial for controlling the ionic environment of these cells.

Duke Scholars

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

March 15, 2001

Volume

20

Issue

6

Start / End Page

1289 / 1299

Location

England

Related Subject Headings

  • Testis
  • Spermatozoa
  • Sertoli Cells
  • Seminiferous Tubules
  • Retinal Degeneration
  • Pigment Epithelium of Eye
  • Photoreceptor Cells, Vertebrate
  • Mice, Knockout
  • Mice
  • Male
 

Citation

APA
Chicago
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MLA
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Bösl, M. R., Stein, V., Hübner, C., Zdebik, A. A., Jordt, S. E., Mukhopadhyay, A. K., … Jentsch, T. J. (2001). Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption. EMBO J, 20(6), 1289–1299. https://doi.org/10.1093/emboj/20.6.1289
Bösl, M. R., V. Stein, C. Hübner, A. A. Zdebik, S. E. Jordt, A. K. Mukhopadhyay, M. S. Davidoff, A. F. Holstein, and T. J. Jentsch. “Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption.EMBO J 20, no. 6 (March 15, 2001): 1289–99. https://doi.org/10.1093/emboj/20.6.1289.
Bösl MR, Stein V, Hübner C, Zdebik AA, Jordt SE, Mukhopadhyay AK, et al. Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption. EMBO J. 2001 Mar 15;20(6):1289–99.
Bösl, M. R., et al. “Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption.EMBO J, vol. 20, no. 6, Mar. 2001, pp. 1289–99. Pubmed, doi:10.1093/emboj/20.6.1289.
Bösl MR, Stein V, Hübner C, Zdebik AA, Jordt SE, Mukhopadhyay AK, Davidoff MS, Holstein AF, Jentsch TJ. Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption. EMBO J. 2001 Mar 15;20(6):1289–1299.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

March 15, 2001

Volume

20

Issue

6

Start / End Page

1289 / 1299

Location

England

Related Subject Headings

  • Testis
  • Spermatozoa
  • Sertoli Cells
  • Seminiferous Tubules
  • Retinal Degeneration
  • Pigment Epithelium of Eye
  • Photoreceptor Cells, Vertebrate
  • Mice, Knockout
  • Mice
  • Male