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Cell-specific aptamers for targeted therapies.

Publication ,  Journal Article
Cerchia, L; Giangrande, PH; McNamara, JO; de Franciscis, V
Published in: Methods Mol Biol
2009

Many signalling proteins involved in diverse functions such as cell growth and differentiation can act as oncogenes and cause cellular transformation. These molecules represent attractive targets for cancer diagnosis or therapy and therefore are subject to intensive investigation. Aptamers are small, highly structured nucleic acid molecules, isolated from combinatorial libraries by a procedure termed SELEX. Aptamers bind to a target molecule by providing a limited number of specific contact points imbedded in a larger, defined three-dimensional structure. Recently, aptamers have been selected against whole living cells, opening a new path which presents three major advantages: (1) direct selection without prior purification of membrane-bound targets, (2) access to membrane proteins in their native conformation similar to the in vivo conditions and (3) identification of (new) targets related to a specific phenotype. The ability to raise aptamers against living cells opens some attractive possibilities for new therapeutic and delivery approaches. In this chapter, the most recent advances in the field will be reviewed together with detailed descriptions of the relevant experimental approaches.

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Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2009

Volume

535

Start / End Page

59 / 78

Location

United States

Related Subject Headings

  • SELEX Aptamer Technique
  • RNA, Small Interfering
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Membrane Proteins
  • Male
  • Humans
  • Drug Delivery Systems
  • Developmental Biology
  • Cytological Techniques
 

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Cerchia, L., Giangrande, P. H., McNamara, J. O., & de Franciscis, V. (2009). Cell-specific aptamers for targeted therapies. Methods Mol Biol, 535, 59–78. https://doi.org/10.1007/978-1-59745-557-2_5
Cerchia, Laura, Paloma H. Giangrande, James O. McNamara, and Vittorio de Franciscis. “Cell-specific aptamers for targeted therapies.Methods Mol Biol 535 (2009): 59–78. https://doi.org/10.1007/978-1-59745-557-2_5.
Cerchia L, Giangrande PH, McNamara JO, de Franciscis V. Cell-specific aptamers for targeted therapies. Methods Mol Biol. 2009;535:59–78.
Cerchia, Laura, et al. “Cell-specific aptamers for targeted therapies.Methods Mol Biol, vol. 535, 2009, pp. 59–78. Pubmed, doi:10.1007/978-1-59745-557-2_5.
Cerchia L, Giangrande PH, McNamara JO, de Franciscis V. Cell-specific aptamers for targeted therapies. Methods Mol Biol. 2009;535:59–78.

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2009

Volume

535

Start / End Page

59 / 78

Location

United States

Related Subject Headings

  • SELEX Aptamer Technique
  • RNA, Small Interfering
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Membrane Proteins
  • Male
  • Humans
  • Drug Delivery Systems
  • Developmental Biology
  • Cytological Techniques