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Encapsulation of stimuli-responsive fusion proteins in silica: Thermally responsive metal ion-sensitive hybrid membranes

Publication ,  Journal Article
Li, L; Im, O; Ashutosh, C; López, GP
Published in: Materials Research Society Symposium Proceedings
December 1, 2013

In this study, we demonstrate the fabrication of hybrid membranes that exhibit discrete and reversible changes in permeability in response to changes in calcium ion (Ca2+) concentration and temperature. Fusion proteins comprising calmodulin (CAM) and elastin-like polypeptides (ELPs) were used as stimuli-responsive elements due to their ability to undergo a reversible lower critical solution temperature (LCST) phase transition, which is sensitive to Ca2+ binding. The calmodulin elastin-like polypeptides fusions (CAM-ELPs) were incorporated into polymerizing silica networks using a simple sol-gel process and spin coating. Permeation experiments with solutions of crystal violet showed that the membranes are both Ca2+-responsive and thermally responsive. Under suitable pressure drop across the membranes, in the absence of Ca2+ or below the LCST of the ELPs, the hybrid membranes are impermeable to water. After addition of Ca2+ or above the LCSTs, they become permeable to water. The permeability can be toggled back and forth by sequential addition of calcium and ethylenediamine tetraacetic acid (EDTA). These results demonstrate that CAM-ELP/silica hybrid membranes can serve as tunable molecular filters whose permeability can be switched on and off in response to Ca2+ and temperature. © 2013 Materials Research Society.

Duke Scholars

Published In

Materials Research Society Symposium Proceedings

DOI

ISSN

0272-9172

Publication Date

December 1, 2013

Volume

1498

Start / End Page

169 / 175
 

Citation

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Li, L., Im, O., Ashutosh, C., & López, G. P. (2013). Encapsulation of stimuli-responsive fusion proteins in silica: Thermally responsive metal ion-sensitive hybrid membranes. Materials Research Society Symposium Proceedings, 1498, 169–175. https://doi.org/10.1557/opl.2013.332
Li, L., O. Im, C. Ashutosh, and G. P. López. “Encapsulation of stimuli-responsive fusion proteins in silica: Thermally responsive metal ion-sensitive hybrid membranes.” Materials Research Society Symposium Proceedings 1498 (December 1, 2013): 169–75. https://doi.org/10.1557/opl.2013.332.
Li L, Im O, Ashutosh C, López GP. Encapsulation of stimuli-responsive fusion proteins in silica: Thermally responsive metal ion-sensitive hybrid membranes. Materials Research Society Symposium Proceedings. 2013 Dec 1;1498:169–75.
Li, L., et al. “Encapsulation of stimuli-responsive fusion proteins in silica: Thermally responsive metal ion-sensitive hybrid membranes.” Materials Research Society Symposium Proceedings, vol. 1498, Dec. 2013, pp. 169–75. Scopus, doi:10.1557/opl.2013.332.
Li L, Im O, Ashutosh C, López GP. Encapsulation of stimuli-responsive fusion proteins in silica: Thermally responsive metal ion-sensitive hybrid membranes. Materials Research Society Symposium Proceedings. 2013 Dec 1;1498:169–175.

Published In

Materials Research Society Symposium Proceedings

DOI

ISSN

0272-9172

Publication Date

December 1, 2013

Volume

1498

Start / End Page

169 / 175