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MIG-10 (lamellipodin) has netrin-independent functions and is a FOS-1A transcriptional target during anchor cell invasion in C. elegans.

Publication ,  Journal Article
Wang, Z; Chi, Q; Sherwood, DR
Published in: Development (Cambridge, England)
March 2014

To transmigrate basement membrane, cells must coordinate distinct signaling activities to breach and pass through this dense extracellular matrix barrier. Netrin expression and activity are strongly associated with invasion in developmental and pathological processes, but how netrin signaling is coordinated with other pathways during invasion is poorly understood. Using the model of anchor cell (AC) invasion in C. elegans, we have previously shown that the integrin receptor heterodimer INA-1/PAT-3 promotes netrin receptor UNC-40 (DCC) localization to the invasive cell membrane of the AC. UNC-6 (netrin)/UNC-40 interactions generate an invasive protrusion that crosses the basement membrane. To understand how UNC-40 signals during invasion, we have used genetic, site of action and live-cell imaging studies to examine the roles of known effectors of UNC-40 signaling in axon outgrowth during AC invasion. UNC-34 (Ena/VASP), the Rac GTPases MIG-2 and CED-10 and the actin binding protein UNC-115 (abLIM) are dedicated UNC-40 effectors that are recruited to the invasive membrane by UNC-40 and generate F-actin. MIG-10 (lamellipodin), an effector of UNC-40 in neurons, however, has independent functions from UNC-6/UNC-40. Furthermore, unlike other UNC-40 effectors, its expression is regulated by FOS-1A, a transcription factor that promotes basement membrane breaching. Similar to UNC-40, however, MIG-10 localization to the invasive cell membrane is also dependent on the integrin INA-1/PAT-3. These studies indicate that MIG-10 has distinct functions from UNC-40 signaling in cell invasion, and demonstrate that integrin coordinates invasion by localizing these molecules to the cell-basement membrane interface.

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Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

March 2014

Volume

141

Issue

6

Start / End Page

1342 / 1353

Related Subject Headings

  • Signal Transduction
  • Proto-Oncogene Proteins c-fos
  • Netrins
  • Nerve Tissue Proteins
  • Integrins
  • Integrin beta Chains
  • Genes, Helminth
  • Gene Expression Regulation, Developmental
  • Cell Movement
  • Cell Adhesion Molecules
 

Citation

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Wang, Z., Chi, Q., & Sherwood, D. R. (2014). MIG-10 (lamellipodin) has netrin-independent functions and is a FOS-1A transcriptional target during anchor cell invasion in C. elegans. Development (Cambridge, England), 141(6), 1342–1353. https://doi.org/10.1242/dev.102434
Wang, Zheng, Qiuyi Chi, and David R. Sherwood. “MIG-10 (lamellipodin) has netrin-independent functions and is a FOS-1A transcriptional target during anchor cell invasion in C. elegans.Development (Cambridge, England) 141, no. 6 (March 2014): 1342–53. https://doi.org/10.1242/dev.102434.
Wang, Zheng, et al. “MIG-10 (lamellipodin) has netrin-independent functions and is a FOS-1A transcriptional target during anchor cell invasion in C. elegans.Development (Cambridge, England), vol. 141, no. 6, Mar. 2014, pp. 1342–53. Epmc, doi:10.1242/dev.102434.
Wang Z, Chi Q, Sherwood DR. MIG-10 (lamellipodin) has netrin-independent functions and is a FOS-1A transcriptional target during anchor cell invasion in C. elegans. Development (Cambridge, England). 2014 Mar;141(6):1342–1353.
Journal cover image

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

March 2014

Volume

141

Issue

6

Start / End Page

1342 / 1353

Related Subject Headings

  • Signal Transduction
  • Proto-Oncogene Proteins c-fos
  • Netrins
  • Nerve Tissue Proteins
  • Integrins
  • Integrin beta Chains
  • Genes, Helminth
  • Gene Expression Regulation, Developmental
  • Cell Movement
  • Cell Adhesion Molecules