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In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment.

Publication ,  Journal Article
Sipkins, DA; Wei, X; Wu, JW; Runnels, JM; Côté, D; Means, TK; Luster, AD; Scadden, DT; Lin, CP
Published in: Nature
June 16, 2005

The organization of cellular niches is known to have a key role in regulating normal stem cell differentiation and regeneration, but relatively little is known about the architecture of microenvironments that support malignant metastasis. Using dynamic in vivo confocal imaging, here we show that murine bone marrow contains unique anatomic regions defined by specialized endothelium. This vasculature expresses the adhesion molecule E-selectin and the chemoattractant stromal-cell-derived factor 1 (SDF-1) in discrete, discontinuous areas that influence the homing of a variety of tumour cell lines. Disruption of the interactions between SDF-1 and its receptor CXCR4 inhibits the homing of Nalm-6 cells (an acute lymphoblastic leukaemia cell line) to these vessels. Further studies revealed that circulating leukaemic cells can engraft around these vessels, suggesting that this molecularly distinct vasculature demarcates a microenvironment for early metastatic tumour spread in bone marrow. Finally, purified haematopoietic stem/progenitor cells and lymphocytes also localize to the same microdomains, indicating that this vasculature might also function in benign states to demarcate specific portals for the entry of cells into the marrow space. Specialized vascular structures therefore appear to delineate a microenvironment with unique physiology that can be exploited by circulating malignant cells.

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Published In

Nature

DOI

EISSN

1476-4687

Publication Date

June 16, 2005

Volume

435

Issue

7044

Start / End Page

969 / 973

Location

England

Related Subject Headings

  • Skull
  • Receptors, CXCR4
  • Neoplasms
  • Microscopy, Confocal
  • Mice, SCID
  • Mice, Inbred BALB C
  • Mice
  • Leukemia
  • General Science & Technology
  • Endothelial Cells
 

Citation

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Chicago
ICMJE
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Sipkins, D. A., Wei, X., Wu, J. W., Runnels, J. M., Côté, D., Means, T. K., … Lin, C. P. (2005). In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment. Nature, 435(7044), 969–973. https://doi.org/10.1038/nature03703
Sipkins, Dorothy A., Xunbin Wei, Juwell W. Wu, Judith M. Runnels, Daniel Côté, Terry K. Means, Andrew D. Luster, David T. Scadden, and Charles P. Lin. “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment.Nature 435, no. 7044 (June 16, 2005): 969–73. https://doi.org/10.1038/nature03703.
Sipkins DA, Wei X, Wu JW, Runnels JM, Côté D, Means TK, et al. In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment. Nature. 2005 Jun 16;435(7044):969–73.
Sipkins, Dorothy A., et al. “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment.Nature, vol. 435, no. 7044, June 2005, pp. 969–73. Pubmed, doi:10.1038/nature03703.
Sipkins DA, Wei X, Wu JW, Runnels JM, Côté D, Means TK, Luster AD, Scadden DT, Lin CP. In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment. Nature. 2005 Jun 16;435(7044):969–973.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

Publication Date

June 16, 2005

Volume

435

Issue

7044

Start / End Page

969 / 973

Location

England

Related Subject Headings

  • Skull
  • Receptors, CXCR4
  • Neoplasms
  • Microscopy, Confocal
  • Mice, SCID
  • Mice, Inbred BALB C
  • Mice
  • Leukemia
  • General Science & Technology
  • Endothelial Cells