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Direct reprogramming of cardiac fibroblasts to cardiomyocytes using microRNAs.

Publication ,  Journal Article
Jayawardena, T; Mirotsou, M; Dzau, VJ
Published in: Methods Mol Biol
2014

The therapeutic administration of microRNAs represents an innovative reprogramming strategy with which to advance cardiac regeneration and personalized medicine. Recently, a distinct set of microRNAs was found capable of converting murine fibroblasts to cardiomyocyte-like cells in vitro. Further treatment with JAK inhibitor I significantly enhanced the efficiency of the microRNA-mediated reprogramming (Jayawardena et al., Circ Res 110(11):1465-1473, 2012). This novel technique serves as an initial tool for switching the cell fate of cardiac fibroblasts toward the cardiomyocyte lineage using microRNAs. As the budding field of reprogramming biology develops, we hope that a thorough examination of the chemical, physical, and temporal parameters determining reprogramming efficiency and maturation will enable a better understanding of the mechanisms governing cardiac cell fate and provide new approaches for drug discovery and therapy for cardiovascular diseases.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2014

Volume

1150

Start / End Page

263 / 272

Location

United States

Related Subject Headings

  • Transfection
  • Myocytes, Cardiac
  • MicroRNAs
  • Mice
  • Heart Ventricles
  • Fibroblasts
  • Developmental Biology
  • DNA, Complementary
  • Cloning, Molecular
  • Cellular Reprogramming
 

Citation

APA
Chicago
ICMJE
MLA
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Jayawardena, T., Mirotsou, M., & Dzau, V. J. (2014). Direct reprogramming of cardiac fibroblasts to cardiomyocytes using microRNAs. Methods Mol Biol, 1150, 263–272. https://doi.org/10.1007/978-1-4939-0512-6_18
Jayawardena, Tilanthi, Maria Mirotsou, and Victor J. Dzau. “Direct reprogramming of cardiac fibroblasts to cardiomyocytes using microRNAs.Methods Mol Biol 1150 (2014): 263–72. https://doi.org/10.1007/978-1-4939-0512-6_18.
Jayawardena T, Mirotsou M, Dzau VJ. Direct reprogramming of cardiac fibroblasts to cardiomyocytes using microRNAs. Methods Mol Biol. 2014;1150:263–72.
Jayawardena, Tilanthi, et al. “Direct reprogramming of cardiac fibroblasts to cardiomyocytes using microRNAs.Methods Mol Biol, vol. 1150, 2014, pp. 263–72. Pubmed, doi:10.1007/978-1-4939-0512-6_18.
Jayawardena T, Mirotsou M, Dzau VJ. Direct reprogramming of cardiac fibroblasts to cardiomyocytes using microRNAs. Methods Mol Biol. 2014;1150:263–272.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2014

Volume

1150

Start / End Page

263 / 272

Location

United States

Related Subject Headings

  • Transfection
  • Myocytes, Cardiac
  • MicroRNAs
  • Mice
  • Heart Ventricles
  • Fibroblasts
  • Developmental Biology
  • DNA, Complementary
  • Cloning, Molecular
  • Cellular Reprogramming