Whole-mount in situ hybridization on murine skeletogenic tissues.
Publication
, Journal Article
Rutkowsky, T; Sharma, D; Hilton, MJ
Published in: Methods Mol Biol
2014
Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2014
Volume
1130
Start / End Page
193 / 201
Location
United States
Related Subject Headings
- Mice
- In Situ Hybridization
- Immunohistochemistry
- Gene Expression
- Fetus
- Developmental Biology
- Bone and Bones
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Rutkowsky, T., Sharma, D., & Hilton, M. J. (2014). Whole-mount in situ hybridization on murine skeletogenic tissues. Methods Mol Biol, 1130, 193–201. https://doi.org/10.1007/978-1-62703-989-5_14
Rutkowsky, Timothy, Deepika Sharma, and Matthew J. Hilton. “Whole-mount in situ hybridization on murine skeletogenic tissues.” Methods Mol Biol 1130 (2014): 193–201. https://doi.org/10.1007/978-1-62703-989-5_14.
Rutkowsky T, Sharma D, Hilton MJ. Whole-mount in situ hybridization on murine skeletogenic tissues. Methods Mol Biol. 2014;1130:193–201.
Rutkowsky, Timothy, et al. “Whole-mount in situ hybridization on murine skeletogenic tissues.” Methods Mol Biol, vol. 1130, 2014, pp. 193–201. Pubmed, doi:10.1007/978-1-62703-989-5_14.
Rutkowsky T, Sharma D, Hilton MJ. Whole-mount in situ hybridization on murine skeletogenic tissues. Methods Mol Biol. 2014;1130:193–201.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2014
Volume
1130
Start / End Page
193 / 201
Location
United States
Related Subject Headings
- Mice
- In Situ Hybridization
- Immunohistochemistry
- Gene Expression
- Fetus
- Developmental Biology
- Bone and Bones
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology