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Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis.

Publication ,  Journal Article
DeFalco, T; Bhattacharya, I; Williams, AV; Sams, DM; Capel, B
Published in: Proc Natl Acad Sci U S A
June 10, 2014

Organogenesis of the testis is initiated when expression of Sry in pre-Sertoli cells directs the gonad toward a male-specific fate. The cells in the early bipotential gonad undergo de novo organization to form testis cords that enclose germ cells inside tubules lined by epithelial Sertoli cells. Although Sertoli cells are a driving force in the de novo formation of testis cords, recent studies in mouse showed that reorganization of the vasculature and of interstitial cells also play critical roles in testis cord morphogenesis. However, the mechanism driving reorganization of the vasculature during fetal organogenesis remained unclear. Here we demonstrate that fetal macrophages are associated with nascent gonadal and mesonephric vasculature during the initial phases of testis morphogenesis. Macrophages mediate vascular reorganization and prune errant germ cells and somatic cells after testis architecture is established. We show that gonadal macrophages are derived from primitive yolk-sac hematopoietic progenitors and exhibit hallmarks of M2 activation status, suggestive of angiogenic and tissue remodeling functions. Depletion of macrophages resulted in impaired vascular reorganization and abnormal cord formation. These findings reveal a previously unappreciated role for macrophages in testis morphogenesis and suggest that macrophages are an intermediary between neovascularization and organ architecture during fetal organogenesis.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

June 10, 2014

Volume

111

Issue

23

Start / End Page

E2384 / E2393

Location

United States

Related Subject Headings

  • Yolk Sac
  • Time Factors
  • Testis
  • Receptors, Chemokine
  • Organ Culture Techniques
  • Myeloid Cells
  • Morphogenesis
  • Microscopy, Confocal
  • Mice, Transgenic
  • Mice, Knockout
 

Citation

APA
Chicago
ICMJE
MLA
NLM
DeFalco, T., Bhattacharya, I., Williams, A. V., Sams, D. M., & Capel, B. (2014). Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis. Proc Natl Acad Sci U S A, 111(23), E2384–E2393. https://doi.org/10.1073/pnas.1400057111
DeFalco, Tony, Indrashis Bhattacharya, Alyna V. Williams, Dustin M. Sams, and Blanche Capel. “Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis.Proc Natl Acad Sci U S A 111, no. 23 (June 10, 2014): E2384–93. https://doi.org/10.1073/pnas.1400057111.
DeFalco T, Bhattacharya I, Williams AV, Sams DM, Capel B. Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):E2384–93.
DeFalco, Tony, et al. “Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis.Proc Natl Acad Sci U S A, vol. 111, no. 23, June 2014, pp. E2384–93. Pubmed, doi:10.1073/pnas.1400057111.
DeFalco T, Bhattacharya I, Williams AV, Sams DM, Capel B. Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):E2384–E2393.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

June 10, 2014

Volume

111

Issue

23

Start / End Page

E2384 / E2393

Location

United States

Related Subject Headings

  • Yolk Sac
  • Time Factors
  • Testis
  • Receptors, Chemokine
  • Organ Culture Techniques
  • Myeloid Cells
  • Morphogenesis
  • Microscopy, Confocal
  • Mice, Transgenic
  • Mice, Knockout