Accelerate sepsis diagnosis by seamless integration of DNA purification and QPCR
Publication
, Journal Article
Hsu, BN; Madison, AC; Fair, RB
Published in: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
January 1, 2012
The high mortality rate of sepsis has been attributed to delayed pathogen identification. Hence, a fluidic device is being developed to accelerate the procedures from DNA purification to qPCR sequence detection. Demonstrated herein is the key feature the device: The use of EWD droplet actuation to manipulate the eluent generated by an immiscible phase filtration DNA preprocessor. Among other benefits, EWD actuation enables the eluent to be compartmentalized into several microdrops for subsequent automated PCR mixture preparation and multiplex sequence detection. Also discussed are the characterization of purification power, and the fabrication of the high temperature tolerance plastic top plate.
Duke Scholars
Published In
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publication Date
January 1, 2012
Start / End Page
830 / 832
Citation
APA
Chicago
ICMJE
MLA
NLM
Hsu, B. N., Madison, A. C., & Fair, R. B. (2012). Accelerate sepsis diagnosis by seamless integration of DNA purification and QPCR. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 830–832.
Hsu, B. N., A. C. Madison, and R. B. Fair. “Accelerate sepsis diagnosis by seamless integration of DNA purification and QPCR.” Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, January 1, 2012, 830–32.
Hsu BN, Madison AC, Fair RB. Accelerate sepsis diagnosis by seamless integration of DNA purification and QPCR. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. 2012 Jan 1;830–2.
Hsu, B. N., et al. “Accelerate sepsis diagnosis by seamless integration of DNA purification and QPCR.” Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Jan. 2012, pp. 830–32.
Hsu BN, Madison AC, Fair RB. Accelerate sepsis diagnosis by seamless integration of DNA purification and QPCR. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. 2012 Jan 1;830–832.
Published In
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publication Date
January 1, 2012
Start / End Page
830 / 832