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Complexities associated with the molecular and proteomic identification of Paecilomyces species in the clinical mycology laboratory.

Publication ,  Journal Article
Barker, AP; Horan, JL; Slechta, ES; Alexander, BD; Hanson, KE
Published in: Med Mycol
July 2014

Paecilomyces species are emerging fungal pathogens. Morphological identifications are complicated by similarities among the members of the P. variotii complex as well as to some Rasamsonia and Hamigera species. The purpose of this study was to compare matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) with molecular diagnostic standards (i.e., multilocus DNA sequencing of the internal transcribed spacer regions 1 and 2, D1/D2 regions, and part of the β-tubulin gene) for the identification of Paecilomyces spp. encountered in two clinical mycology laboratories. A total of 77 clinical isolates identified morphologically as P. variotii (n = 21), P. lilacinus (n = 52), and Paecilomyces spp. not otherwise specified (n = 4) were included. In accord with the most recent taxonomy, all P. lilacinus isolates were confirmed as Purpureocillium lilacinum by both sequencing and MALDI-TOF MS. Fungi phenotypically resembling P. variotii or Paecilomyces spp. were identified by molecular techniques as P. variotii sensu stricto (n = 12), P. formosus (n = 3), P. dactylethromorphus (n = 3), Rasamsonia argillacea (n = 4), or R. piperina (n = 1) and at the genus level as an isolate of a Hamigera sp. and a Paecilomyces sp. There was 92.2% (71/77) agreement between the molecular and proteomic methods only after supplementation of the MALDI-TOF MS database with type strains. Paecilomyces variotii-like organisms required multilocus DNA interrogations for differentiation and account for all of the fungi whose identification was missed by MALDI-TOF MS. Overall, MALDI-TOF MS was a rapid and reliable alternative to multilocus sequencing. However, significant augmentation of the commercially available database was required to reproducibly identify this group of important human pathogens.

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Published In

Med Mycol

DOI

EISSN

1460-2709

Publication Date

July 2014

Volume

52

Issue

5

Start / End Page

537 / 545

Location

England

Related Subject Headings

  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Reproducibility of Results
  • Proteomics
  • Principal Component Analysis
  • Phylogeny
  • Paecilomyces
  • Mycoses
  • Mycological Typing Techniques
  • Multilocus Sequence Typing
  • Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Barker, A. P., Horan, J. L., Slechta, E. S., Alexander, B. D., & Hanson, K. E. (2014). Complexities associated with the molecular and proteomic identification of Paecilomyces species in the clinical mycology laboratory. Med Mycol, 52(5), 537–545. https://doi.org/10.1093/mmy/myu001
Barker, Adam P., Jennifer L. Horan, E Susan Slechta, Barbara D. Alexander, and Kimberly E. Hanson. “Complexities associated with the molecular and proteomic identification of Paecilomyces species in the clinical mycology laboratory.Med Mycol 52, no. 5 (July 2014): 537–45. https://doi.org/10.1093/mmy/myu001.
Barker AP, Horan JL, Slechta ES, Alexander BD, Hanson KE. Complexities associated with the molecular and proteomic identification of Paecilomyces species in the clinical mycology laboratory. Med Mycol. 2014 Jul;52(5):537–45.
Barker, Adam P., et al. “Complexities associated with the molecular and proteomic identification of Paecilomyces species in the clinical mycology laboratory.Med Mycol, vol. 52, no. 5, July 2014, pp. 537–45. Pubmed, doi:10.1093/mmy/myu001.
Barker AP, Horan JL, Slechta ES, Alexander BD, Hanson KE. Complexities associated with the molecular and proteomic identification of Paecilomyces species in the clinical mycology laboratory. Med Mycol. 2014 Jul;52(5):537–545.
Journal cover image

Published In

Med Mycol

DOI

EISSN

1460-2709

Publication Date

July 2014

Volume

52

Issue

5

Start / End Page

537 / 545

Location

England

Related Subject Headings

  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Reproducibility of Results
  • Proteomics
  • Principal Component Analysis
  • Phylogeny
  • Paecilomyces
  • Mycoses
  • Mycological Typing Techniques
  • Multilocus Sequence Typing
  • Microbiology