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A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate.

Publication ,  Journal Article
Walsh, DI; Sommer, GJ; Schaff, UY; Hahn, PS; Jaffe, GJ; Murthy, SK
Published in: Lab Chip
August 7, 2014

We present a novel "Lab-on-a-Disk" platform and demonstrate its capability for rapid and sensitive measurement of vascular endothelial growth factor (VEGF) intended for patients suffering from diabetic retinopathy (DR) and age-related macular degeneration (AMD). This approach combines sedimentation principles applied to microspheres under centrifugal force with signal amplification using an enzyme and a fluorogenic substrate for readout. The simple single channel per assay platform separates, washes and concentrates antibody-coated microspheres from excess label to produce a sensitive fluorogenic response proportional to the amount of VEGF in the sample. This platform has comparable sensitivity to conventional ELISA and can generate a readout within 16-18 min with no sample preparation beyond mixing assay reagents and loading on the disk. In the context of ocular diagnostics, this device has the potential to facilitate accurate dosing of anti-VEGF medications utilized to treat DR and AMD, as well as identify patients whose ocular VEGF levels are not elevated and who would therefore not benefit from standard anti-VEGF medications.

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Published In

Lab Chip

DOI

EISSN

1473-0189

Publication Date

August 7, 2014

Volume

14

Issue

15

Start / End Page

2673 / 2680

Location

England

Related Subject Headings

  • Wet Macular Degeneration
  • Vascular Endothelial Growth Factor A
  • Time Factors
  • Sensitivity and Specificity
  • Point-of-Care Systems
  • Microspheres
  • Microfluidic Analytical Techniques
  • Microchemistry
  • Materials Testing
  • Macular Edema
 

Citation

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ICMJE
MLA
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Walsh, D. I., Sommer, G. J., Schaff, U. Y., Hahn, P. S., Jaffe, G. J., & Murthy, S. K. (2014). A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate. Lab Chip, 14(15), 2673–2680. https://doi.org/10.1039/c4lc00279b
Walsh, David I., Gregory J. Sommer, Ulrich Y. Schaff, Paul S. Hahn, Glenn J. Jaffe, and Shashi K. Murthy. “A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate.Lab Chip 14, no. 15 (August 7, 2014): 2673–80. https://doi.org/10.1039/c4lc00279b.
Walsh DI, Sommer GJ, Schaff UY, Hahn PS, Jaffe GJ, Murthy SK. A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate. Lab Chip. 2014 Aug 7;14(15):2673–80.
Walsh, David I., et al. “A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate.Lab Chip, vol. 14, no. 15, Aug. 2014, pp. 2673–80. Pubmed, doi:10.1039/c4lc00279b.
Walsh DI, Sommer GJ, Schaff UY, Hahn PS, Jaffe GJ, Murthy SK. A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate. Lab Chip. 2014 Aug 7;14(15):2673–2680.
Journal cover image

Published In

Lab Chip

DOI

EISSN

1473-0189

Publication Date

August 7, 2014

Volume

14

Issue

15

Start / End Page

2673 / 2680

Location

England

Related Subject Headings

  • Wet Macular Degeneration
  • Vascular Endothelial Growth Factor A
  • Time Factors
  • Sensitivity and Specificity
  • Point-of-Care Systems
  • Microspheres
  • Microfluidic Analytical Techniques
  • Microchemistry
  • Materials Testing
  • Macular Edema