A study of axonal protein trafficking in neuronal networks via the microfluidic platform
Publication
, Journal Article
Fu, Y; Vandongen, A; Bourouina, T; Tsang, WM; Je, M; Liu, AQ
Published in: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012
January 1, 2012
This paper reports a microfluidic platform for culturing neuronal networks and the study of axonal protein trafficking. The device consists of cultural microchambers connected by microchannel array. Molecular biological methods were used to express fluorescent proteins in neurons. Preliminary results show that the neurons can be polarized with their soma and axons being compartmentalized into different fluidically isolated microenvironments. When chemical stimulation was applied to axonal chamber, anterograde migration of expressed fluorescent proteins could be observed. The platform will be useful in neuroscience research for studying the response of individual neurons to extracellular stimulation.
Duke Scholars
Published In
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012
Publication Date
January 1, 2012
Start / End Page
1072 / 1074
Citation
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ICMJE
MLA
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Fu, Y., Vandongen, A., Bourouina, T., Tsang, W. M., Je, M., & Liu, A. Q. (2012). A study of axonal protein trafficking in neuronal networks via the microfluidic platform. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012, 1072–1074.
Fu, Y., A. Vandongen, T. Bourouina, W. M. Tsang, M. Je, and A. Q. Liu. “A study of axonal protein trafficking in neuronal networks via the microfluidic platform.” Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012, January 1, 2012, 1072–74.
Fu Y, Vandongen A, Bourouina T, Tsang WM, Je M, Liu AQ. A study of axonal protein trafficking in neuronal networks via the microfluidic platform. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012. 2012 Jan 1;1072–4.
Fu, Y., et al. “A study of axonal protein trafficking in neuronal networks via the microfluidic platform.” Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012, Jan. 2012, pp. 1072–74.
Fu Y, Vandongen A, Bourouina T, Tsang WM, Je M, Liu AQ. A study of axonal protein trafficking in neuronal networks via the microfluidic platform. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012. 2012 Jan 1;1072–1074.
Published In
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012
Publication Date
January 1, 2012
Start / End Page
1072 / 1074