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Renal epithelial cells produce and spread HIV-1 via T-cell contact.

Publication ,  Journal Article
Blasi, M; Balakumaran, B; Chen, P; Negri, DRM; Cara, A; Chen, BK; Klotman, ME
Published in: AIDS
October 23, 2014

OBJECTIVES: Increasing evidence supports the role of the kidney as a reservoir for HIV-1. In-vitro co-cultivation of HIV-infected T cells with renal tubule epithelial (RTE) cells results in virus transfer to the latter, whereas cell-free virus infection is inefficient. We further characterized the fate of HIV-1 after it is internalized in renal epithelial cells. METHODS: Primary or immortalized CD4 cells were infected with a green fluorescent protein (GFP)-expressing replication competent HIV-1. HIV-1 transfer from T cells to RTE cells was carried out in a co-culture system and evaluated by fluorescence-activated cell sorting analysis. HIV-1 integration in renal cells was evaluated by Alu-PCR and the production of infectious particles was assessed by p24-ELISA and TZM-bl assay. HIV-infected renal cells were used as donor cells in a co-culture system to evaluate their ability to transfer the virus back to T cells. RESULTS: Renal cells become productively infected by HIV-1 and multiple copies of HIV-1 can be transferred from infected T cells to renal cells. Two separate cell populations were identified among infected renal cells based on reporter gene GFP expression level (low vs. high), only the high showing sensitivity to azidothymidine and ritonavir. Co-cultivation of HIV-1-infected renal cells with noninfected T cells resulted in HIV-1 transmission to T cells, supporting bidirectional exchange of virus between T cells and kidney-derived cells. Persistent expression and generation of infectious virus in renal cells required HIV integration. CONCLUSION: These results support the kidney as a potential reservoir where virus is exchanged between interstitial T cells and RTE cells.

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Published In

AIDS

DOI

EISSN

1473-5571

Publication Date

October 23, 2014

Volume

28

Issue

16

Start / End Page

2345 / 2353

Location

England

Related Subject Headings

  • Virus Replication
  • Virus Integration
  • Virology
  • T-Lymphocytes
  • Kidney
  • Humans
  • HIV-1
  • HIV Core Protein p24
  • Epithelial Cells
  • Enzyme-Linked Immunosorbent Assay
 

Citation

APA
Chicago
ICMJE
MLA
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Blasi, M., Balakumaran, B., Chen, P., Negri, D. R. M., Cara, A., Chen, B. K., & Klotman, M. E. (2014). Renal epithelial cells produce and spread HIV-1 via T-cell contact. AIDS, 28(16), 2345–2353. https://doi.org/10.1097/QAD.0000000000000398
Blasi, Maria, Bala Balakumaran, Ping Chen, Donatella R. M. Negri, Andrea Cara, Benjamin K. Chen, and Mary E. Klotman. “Renal epithelial cells produce and spread HIV-1 via T-cell contact.AIDS 28, no. 16 (October 23, 2014): 2345–53. https://doi.org/10.1097/QAD.0000000000000398.
Blasi M, Balakumaran B, Chen P, Negri DRM, Cara A, Chen BK, et al. Renal epithelial cells produce and spread HIV-1 via T-cell contact. AIDS. 2014 Oct 23;28(16):2345–53.
Blasi, Maria, et al. “Renal epithelial cells produce and spread HIV-1 via T-cell contact.AIDS, vol. 28, no. 16, Oct. 2014, pp. 2345–53. Pubmed, doi:10.1097/QAD.0000000000000398.
Blasi M, Balakumaran B, Chen P, Negri DRM, Cara A, Chen BK, Klotman ME. Renal epithelial cells produce and spread HIV-1 via T-cell contact. AIDS. 2014 Oct 23;28(16):2345–2353.

Published In

AIDS

DOI

EISSN

1473-5571

Publication Date

October 23, 2014

Volume

28

Issue

16

Start / End Page

2345 / 2353

Location

England

Related Subject Headings

  • Virus Replication
  • Virus Integration
  • Virology
  • T-Lymphocytes
  • Kidney
  • Humans
  • HIV-1
  • HIV Core Protein p24
  • Epithelial Cells
  • Enzyme-Linked Immunosorbent Assay