Skip to main content
Journal cover image

Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model.

Publication ,  Journal Article
McNamara, ER; Madden-Fuentes, RJ; Routh, JC; Rouse, D; Madden, JF; Wiener, JS; Rushton, HG; Ross, SS
Published in: J Pediatr Urol
August 2014

OBJECTIVE: We hypothesized that cold ischemia during partial orchiectomy would lead to higher serum testosterone levels and preservation of testicular architecture than warm ischemia in a prepubescent rat model. MATERIALS AND METHODS: Eighteen prepubescent male Sprague-Dawley rats were randomized to three different surgical groups: sham surgery, bilateral partial orchiectomy with 30 min of cord compression with cold ischemia, or bilateral partial orchiectomy with 30 min of cord compression with warm ischemia. Animals were killed at puberty, and serum, sperm, and testicles were collected. Histological tissue injury was graded by standardized methodology. RESULTS: Mean serum testosterone levels were 1445 ± 590 pg/mL for the sham group, 449 ± 268 pg/mL for the cold ischemia group and 879 ± 631 pg/mL for the warm ischemia group (p = 0.12). Mean sperm counts were 2.1 × 10(7) for sham, 4.4 × 10(6) for cold ischemia, and 9.9 × 10(6) for the warm ischemia groups (p = 0.48). Histological evaluation revealed significant difference in tissue injury grading with more injury in the cold ischemia than in the warm ischemia group (p = 0.01). CONCLUSIONS: In our preclinical rat model, we found no benefit for cold ischemia over warm ischemia at 30 min.

Duke Scholars

Published In

J Pediatr Urol

DOI

EISSN

1873-4898

Publication Date

August 2014

Volume

10

Issue

4

Start / End Page

593 / 597

Location

England

Related Subject Headings

  • Warm Ischemia
  • Urology & Nephrology
  • Testosterone
  • Testis
  • Sperm Count
  • Sexual Maturation
  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Orchiectomy
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McNamara, E. R., Madden-Fuentes, R. J., Routh, J. C., Rouse, D., Madden, J. F., Wiener, J. S., … Ross, S. S. (2014). Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model. J Pediatr Urol, 10(4), 593–597. https://doi.org/10.1016/j.jpurol.2014.06.005
McNamara, Erin R., Ramiro J. Madden-Fuentes, Jonathan C. Routh, Douglas Rouse, John F. Madden, John S. Wiener, Harry G. Rushton, and Sherry S. Ross. “Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model.J Pediatr Urol 10, no. 4 (August 2014): 593–97. https://doi.org/10.1016/j.jpurol.2014.06.005.
McNamara ER, Madden-Fuentes RJ, Routh JC, Rouse D, Madden JF, Wiener JS, et al. Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model. J Pediatr Urol. 2014 Aug;10(4):593–7.
McNamara, Erin R., et al. “Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model.J Pediatr Urol, vol. 10, no. 4, Aug. 2014, pp. 593–97. Pubmed, doi:10.1016/j.jpurol.2014.06.005.
McNamara ER, Madden-Fuentes RJ, Routh JC, Rouse D, Madden JF, Wiener JS, Rushton HG, Ross SS. Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model. J Pediatr Urol. 2014 Aug;10(4):593–597.
Journal cover image

Published In

J Pediatr Urol

DOI

EISSN

1873-4898

Publication Date

August 2014

Volume

10

Issue

4

Start / End Page

593 / 597

Location

England

Related Subject Headings

  • Warm Ischemia
  • Urology & Nephrology
  • Testosterone
  • Testis
  • Sperm Count
  • Sexual Maturation
  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Orchiectomy