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Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes

Publication ,  Journal Article
Weinberger, A; Walter, V; MacEwan, SR; Schmatko, T; Muller, P; Schroder, AP; Chilkoti, A; Marques, CM
Published in: Scientific Reports
March 6, 2017

Although cationic cell-penetrating peptides (CPPs) are able to bind to cell membranes, thus promoting cell internalization by active pathways, attachment of cargo molecules to CPPs invariably reduces their cellular uptake. We show here that CPP binding to lipid bilayers, a simple model of the cell membrane, can be recovered by designing cargo molecules that self-assemble into spherical micelles and increase the local interfacial density of CPP on the surface of the cargo. Experiments performed on model giant unilamellar vesicles under a confocal laser scanning microscope show that a family of thermally responsive elastin-like polypeptides that exhibit temperature-triggered micellization can promote temperature triggered attachment of the micelles to membranes, thus rescuing by self-assembly the cargo-induced loss of the CPP affinity to bio-membranes.

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Published In

Scientific Reports

DOI

EISSN

2045-2322

Publication Date

March 6, 2017

Volume

7
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Weinberger, A., Walter, V., MacEwan, S. R., Schmatko, T., Muller, P., Schroder, A. P., … Marques, C. M. (2017). Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes. Scientific Reports, 7. https://doi.org/10.1038/srep43963
Weinberger, A., V. Walter, S. R. MacEwan, T. Schmatko, P. Muller, A. P. Schroder, A. Chilkoti, and C. M. Marques. “Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes.” Scientific Reports 7 (March 6, 2017). https://doi.org/10.1038/srep43963.
Weinberger A, Walter V, MacEwan SR, Schmatko T, Muller P, Schroder AP, et al. Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes. Scientific Reports. 2017 Mar 6;7.
Weinberger, A., et al. “Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes.” Scientific Reports, vol. 7, Mar. 2017. Scopus, doi:10.1038/srep43963.
Weinberger A, Walter V, MacEwan SR, Schmatko T, Muller P, Schroder AP, Chilkoti A, Marques CM. Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes. Scientific Reports. 2017 Mar 6;7.

Published In

Scientific Reports

DOI

EISSN

2045-2322

Publication Date

March 6, 2017

Volume

7