Skip to main content
Journal cover image

Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size.

Publication ,  Journal Article
Marchetti, M; Shaffer, MSP; Zambianchi, M; Chen, S; Superti, F; Schwander, S; Gow, A; Zhang, JJ; Chung, KF; Ryan, MP; Porter, AE; Tetley, TD
Published in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences
February 2015

The alveolar respiratory unit constitutes one of the main targets of inhaled nanoparticles; the effect of engineered nanomaterials (NMs) on human health is largely unknown. Surfactant protein D (SP-D) is synthesized by alveolar type II epithelial cells and released into respiratory secretions; its main function is in immune defence, notably against inhaled microbes. SP-D also plays an important role in modulating an appropriate inflammatory response in the lung, and reduced SP-D is associated with a number of inflammatory lung diseases. Adsorption of SP-D to inhaled NMs may facilitate their removal via macrophage phagocytosis. This study addresses the hypothesis that the chemistry, size and surface modification of engineered NMs will impact on their interaction with, and adsorption of, SP-D. To this purpose, we have examined the interactions between SP-D in human lung lavage and two NMs, carbon nanotubes and polystyrene nanoparticles, with different surface functionalization. We have demonstrated that particle size, functionalization and concentration affect the adsorption of SP-D from human lung lavage. Functionalization with negatively charged groups enhanced the amount of SP-D binding. While SP-D binding would be expected to enhance macrophage phagocytosis, these results suggest that the degree of binding is markedly affected by the physicochemistry of the NM and that deposition of high levels of some nanoparticles within the alveolar unit might deplete SP-D levels and affect alveolar immune defence mechanisms.

Duke Scholars

Published In

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

DOI

EISSN

1471-2970

ISSN

0962-8436

Publication Date

February 2015

Volume

370

Issue

1661

Start / End Page

20140038

Related Subject Headings

  • Surface Properties
  • Pulmonary Surfactant-Associated Protein D
  • Protein Binding
  • Polystyrenes
  • Particle Size
  • Nanotubes, Carbon
  • Nanoparticles
  • Humans
  • Evolutionary Biology
  • Bronchoalveolar Lavage Fluid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Marchetti, M., Shaffer, M. S. P., Zambianchi, M., Chen, S., Superti, F., Schwander, S., … Tetley, T. D. (2015). Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 370(1661), 20140038. https://doi.org/10.1098/rstb.2014.0038
Marchetti, Magda, Milo S. P. Shaffer, Martina Zambianchi, Shu Chen, Fabiana Superti, Stephan Schwander, Andrew Gow, et al. “Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size.Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 370, no. 1661 (February 2015): 20140038. https://doi.org/10.1098/rstb.2014.0038.
Marchetti M, Shaffer MSP, Zambianchi M, Chen S, Superti F, Schwander S, et al. Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size. Philosophical transactions of the Royal Society of London Series B, Biological sciences. 2015 Feb;370(1661):20140038.
Marchetti, Magda, et al. “Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size.Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, vol. 370, no. 1661, Feb. 2015, p. 20140038. Epmc, doi:10.1098/rstb.2014.0038.
Marchetti M, Shaffer MSP, Zambianchi M, Chen S, Superti F, Schwander S, Gow A, Zhang JJ, Chung KF, Ryan MP, Porter AE, Tetley TD. Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size. Philosophical transactions of the Royal Society of London Series B, Biological sciences. 2015 Feb;370(1661):20140038.
Journal cover image

Published In

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

DOI

EISSN

1471-2970

ISSN

0962-8436

Publication Date

February 2015

Volume

370

Issue

1661

Start / End Page

20140038

Related Subject Headings

  • Surface Properties
  • Pulmonary Surfactant-Associated Protein D
  • Protein Binding
  • Polystyrenes
  • Particle Size
  • Nanotubes, Carbon
  • Nanoparticles
  • Humans
  • Evolutionary Biology
  • Bronchoalveolar Lavage Fluid