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Porcine CD80: cloning, characterization, and evidence for its role in direct human T-cell activation.

Publication ,  Journal Article
Tadaki, DK; Williams, A; Lee, KP; Kirk, AD; Harlan, DM
Published in: Xenotransplantation
May 2003

Previous studies has shown that human anti-pig reactivity in mixed lymphocyte cultures require the indirect presentation of antigens by human antigen presenting cells (APC). Xenoreactivity was inhibited by blockade of human costimulatory molecules. We investigated the role of porcine costimulatory molecules in their ability to activate human T cells directly. Porcine CD80 was cloned from lipopolysaccharide (LPS)-activated porcine lymphocytes. Sequence analysis showed a high degree of conservation in residues involved in CD28/CTLA4. COS cells transfected with porcine CD80 was able to activate human T cells in a cyclosporine independent manner, demonstrating that porcine CD80 can costimulate human T cells. Tumor necrosis factor-alpha (TNF-alpha) activated porcine splenocytes have been shown to up-regulate B7s. In order to test the effect of costimulation blockade in a xeno system, activated splenocytes were cultured with purified CD4+ T cells. The results demonstrated that these cells were capable of activating human T cells and this activation can be blocked by using an antihuman CD80 antibody that demonstrated cross-reactivity to porcine CD80. Non-cross reactive antibodies had no effect, again suggesting direct activation of the human T cells. These data suggest that a reagent that can block both the direct and indirect activation is necessary for a discordant xenotransplant.

Duke Scholars

Published In

Xenotransplantation

DOI

ISSN

0908-665X

Publication Date

May 2003

Volume

10

Issue

3

Start / End Page

252 / 258

Location

Denmark

Related Subject Headings

  • Transplantation, Heterologous
  • Transfection
  • T-Lymphocytes
  • Swine
  • Surgery
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Recombinant Proteins
  • Protein Biosynthesis
  • Polymerase Chain Reaction
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tadaki, D. K., Williams, A., Lee, K. P., Kirk, A. D., & Harlan, D. M. (2003). Porcine CD80: cloning, characterization, and evidence for its role in direct human T-cell activation. Xenotransplantation, 10(3), 252–258. https://doi.org/10.1034/j.1399-3089.2003.02004.x
Tadaki, D. K., A. Williams, K. P. Lee, A. D. Kirk, and D. M. Harlan. “Porcine CD80: cloning, characterization, and evidence for its role in direct human T-cell activation.Xenotransplantation 10, no. 3 (May 2003): 252–58. https://doi.org/10.1034/j.1399-3089.2003.02004.x.
Tadaki DK, Williams A, Lee KP, Kirk AD, Harlan DM. Porcine CD80: cloning, characterization, and evidence for its role in direct human T-cell activation. Xenotransplantation. 2003 May;10(3):252–8.
Tadaki, D. K., et al. “Porcine CD80: cloning, characterization, and evidence for its role in direct human T-cell activation.Xenotransplantation, vol. 10, no. 3, May 2003, pp. 252–58. Pubmed, doi:10.1034/j.1399-3089.2003.02004.x.
Tadaki DK, Williams A, Lee KP, Kirk AD, Harlan DM. Porcine CD80: cloning, characterization, and evidence for its role in direct human T-cell activation. Xenotransplantation. 2003 May;10(3):252–258.

Published In

Xenotransplantation

DOI

ISSN

0908-665X

Publication Date

May 2003

Volume

10

Issue

3

Start / End Page

252 / 258

Location

Denmark

Related Subject Headings

  • Transplantation, Heterologous
  • Transfection
  • T-Lymphocytes
  • Swine
  • Surgery
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Recombinant Proteins
  • Protein Biosynthesis
  • Polymerase Chain Reaction