Skip to main content

BK virus and SV40 co-infection in polyomavirus nephropathy.

Publication ,  Journal Article
Li, R-M; Mannon, RB; Kleiner, D; Tsokos, M; Bynum, M; Kirk, AD; Kopp, JB
Published in: Transplantation
December 15, 2002

BACKGROUND: Polyomavirus (PV) nephropathy has been attributed to reactivation of BK virus (BKV) or more rarely JC virus (JCV). The simian virus (SV) 40 is PV that was likely introduced into the human population through contaminated vaccines. The purpose of this study was to identify and characterize the PV that is associated with PV nephropathy. METHODS: The clinical diagnosis of PV nephropathy (PVN) was made in patients with acute deterioration in renal function whose renal biopsies showed typical viral cytopathic changes in tubular epithelial cells and staining for PV T antigen. Polymerase chain reaction (PCR) amplification of DNA from peripheral blood mononuclear cells (PBMC), urinary cells, and renal biopsy tissue was performed using specific primers for the transcription control regions of BKV, JCV, and SV40, respectively. RESULTS: Six cases of PV nephropathy were identified in 91 renal transplant recipients (7%). Immunosuppressive therapy was modified in all patients. Renal function stabilized or improved in four patients and deteriorated in two patients, and one patient has lost his allograft, after follow-up from 2 to 25 months. PCR detection demonstrated BKV genome in three of five PBMC samples, six of six urinary cell samples, and two of four renal biopsies. SV40 genome was detected in two of five PBMC samples, one of six urinary cell samples, and two of four renal biopsies. Infectious SV40 and BKV was demonstrated in CV-1 co-cultures using urine from one patient. JCV was not detected in any PVN sample. Co-infection with BKV and SV40 was found in two PVN patients. Urine samples obtained 12 months after transplant from 26 transplant recipients without PVN on simultaneous protocol renal biopsy were analyzed by PCR; BKV genome was demonstrated in 5 of 25 samples, JCV genome was demonstrated in 3 of 25 samples, and SV40 genome was demonstrated in 0 of 25 samples. CONCLUSION: The authors report molecular evidence that co-infection with BKV and SV40 occurs in renal transplant patients with PVN, suggesting that SV40 may contribute to PVN after renal transplant.

Duke Scholars

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

December 15, 2002

Volume

74

Issue

11

Start / End Page

1497 / 1504

Location

United States

Related Subject Headings

  • Virus Replication
  • Tumor Virus Infections
  • Transplantation, Homologous
  • Surgery
  • Simian virus 40
  • Postoperative Complications
  • Polyomavirus Infections
  • Middle Aged
  • Male
  • Kidney Transplantation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, R.-M., Mannon, R. B., Kleiner, D., Tsokos, M., Bynum, M., Kirk, A. D., & Kopp, J. B. (2002). BK virus and SV40 co-infection in polyomavirus nephropathy. Transplantation, 74(11), 1497–1504. https://doi.org/10.1097/00007890-200212150-00004
Li, Rui-Mei, Roslyn B. Mannon, David Kleiner, Maria Tsokos, Michelle Bynum, Allen D. Kirk, and Jeffrey B. Kopp. “BK virus and SV40 co-infection in polyomavirus nephropathy.Transplantation 74, no. 11 (December 15, 2002): 1497–1504. https://doi.org/10.1097/00007890-200212150-00004.
Li R-M, Mannon RB, Kleiner D, Tsokos M, Bynum M, Kirk AD, et al. BK virus and SV40 co-infection in polyomavirus nephropathy. Transplantation. 2002 Dec 15;74(11):1497–504.
Li, Rui-Mei, et al. “BK virus and SV40 co-infection in polyomavirus nephropathy.Transplantation, vol. 74, no. 11, Dec. 2002, pp. 1497–504. Pubmed, doi:10.1097/00007890-200212150-00004.
Li R-M, Mannon RB, Kleiner D, Tsokos M, Bynum M, Kirk AD, Kopp JB. BK virus and SV40 co-infection in polyomavirus nephropathy. Transplantation. 2002 Dec 15;74(11):1497–1504.

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

December 15, 2002

Volume

74

Issue

11

Start / End Page

1497 / 1504

Location

United States

Related Subject Headings

  • Virus Replication
  • Tumor Virus Infections
  • Transplantation, Homologous
  • Surgery
  • Simian virus 40
  • Postoperative Complications
  • Polyomavirus Infections
  • Middle Aged
  • Male
  • Kidney Transplantation