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Inhibitory effect of hydrogen sulfide on ozone-induced airway inflammation, oxidative stress, and bronchial hyperresponsiveness.

Publication ,  Journal Article
Zhang, P; Li, F; Wiegman, CH; Zhang, M; Hong, Y; Gong, J; Chang, Y; Zhang, JJ; Adcock, I; Chung, KF; Zhou, X
Published in: American journal of respiratory cell and molecular biology
January 2015

Exposure to ozone has been associated with airway inflammation, oxidative stress, and bronchial hyperresponsiveness. The goal of this study was to examine whether these adverse effects of ozone could be prevented or reversed by hydrogen sulfide (H2S) as a reducing agent. The H2S donor sodium (NaHS) (2 mg/kg) or vehicle (PBS) was intraperitoneally injected into mice 1 hour before and after 3-hour ozone (2.5 ppm) or air exposure, and the mice were studied 24 hours later. Preventive and therapeutic treatment with NaHS reduced the ozone-induced increases in the total cells, including neutrophils and macrophages; this treatment also reduced levels of cytokines, including TNF-α, chemokine (C-X-C motif) ligand 1, IL-6, and IL-1β levels in bronchial alveolar lavage fluid; inhibited bronchial hyperresponsiveness; and attenuated ozone-induced increases in total malondialdehyde in bronchoalveolar lavage fluid and decreases in the ratio of reduced glutathione/oxidized glutathione in the lung. Ozone exposure led to decreases in the H2S production rate and in mRNA and protein levels of cystathionine-β-synthetase and cystathionine-γ-lyase in the lung. These effects were prevented and reversed by NaHS treatment. Furthermore, NaHS prevented and reversed the phosphorylation of p38 mitogen-activated protein kinase and heat shock protein 27. H2S may have preventive and therapeutic value in the treatment of airway diseases that have an oxidative stress basis.

Duke Scholars

Published In

American journal of respiratory cell and molecular biology

DOI

EISSN

1535-4989

ISSN

1044-1549

Publication Date

January 2015

Volume

52

Issue

1

Start / End Page

129 / 137

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Respiratory System
  • RNA, Messenger
  • Ozone
  • Oxidative Stress
  • Oxidants, Photochemical
  • Mice
  • Malondialdehyde
  • Male
  • Inflammation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, P., Li, F., Wiegman, C. H., Zhang, M., Hong, Y., Gong, J., … Zhou, X. (2015). Inhibitory effect of hydrogen sulfide on ozone-induced airway inflammation, oxidative stress, and bronchial hyperresponsiveness. American Journal of Respiratory Cell and Molecular Biology, 52(1), 129–137. https://doi.org/10.1165/rcmb.2013-0415oc
Zhang, Pengyu, Feng Li, Coen H. Wiegman, Min Zhang, Yan Hong, Jicheng Gong, Yan Chang, et al. “Inhibitory effect of hydrogen sulfide on ozone-induced airway inflammation, oxidative stress, and bronchial hyperresponsiveness.American Journal of Respiratory Cell and Molecular Biology 52, no. 1 (January 2015): 129–37. https://doi.org/10.1165/rcmb.2013-0415oc.
Zhang P, Li F, Wiegman CH, Zhang M, Hong Y, Gong J, et al. Inhibitory effect of hydrogen sulfide on ozone-induced airway inflammation, oxidative stress, and bronchial hyperresponsiveness. American journal of respiratory cell and molecular biology. 2015 Jan;52(1):129–37.
Zhang, Pengyu, et al. “Inhibitory effect of hydrogen sulfide on ozone-induced airway inflammation, oxidative stress, and bronchial hyperresponsiveness.American Journal of Respiratory Cell and Molecular Biology, vol. 52, no. 1, Jan. 2015, pp. 129–37. Epmc, doi:10.1165/rcmb.2013-0415oc.
Zhang P, Li F, Wiegman CH, Zhang M, Hong Y, Gong J, Chang Y, Zhang JJ, Adcock I, Chung KF, Zhou X. Inhibitory effect of hydrogen sulfide on ozone-induced airway inflammation, oxidative stress, and bronchial hyperresponsiveness. American journal of respiratory cell and molecular biology. 2015 Jan;52(1):129–137.

Published In

American journal of respiratory cell and molecular biology

DOI

EISSN

1535-4989

ISSN

1044-1549

Publication Date

January 2015

Volume

52

Issue

1

Start / End Page

129 / 137

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Respiratory System
  • RNA, Messenger
  • Ozone
  • Oxidative Stress
  • Oxidants, Photochemical
  • Mice
  • Malondialdehyde
  • Male
  • Inflammation