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Split tolerance induced by immunotoxin in a rhesus kidney allograft model.

Publication ,  Journal Article
Fechner, JH; Vargo, DJ; Geissler, EK; Graeb, C; Wang, J; Hanaway, MJ; Watkins, DI; Piekarczyk, M; Neville, DM; Knechtle, SJ
Published in: Transplantation
May 15, 1997

BACKGROUND: Renal allografts were performed in rhesus monkeys using FN18-CRM9, a potent immunotoxin capable of depleting T cells to less than 1% of baseline levels in blood and lymph nodes, as a preparative agent. We have recently reported that animals pretreated with FN18-CRM9 1 week before transplantation without further immunosuppression had prolonged graft survival time compared with control animals, and frequently became tolerant. METHODS: This report examines the alloimmune responses of recipient monkeys to the donor, including cytotoxic T lymphocyte precursor (CTLp) frequency, mixed lymphocyte response, and antidonor IgG response. RESULTS: CTLp frequencies declined significantly (P<0.01) after FN18-CRM9 treatment and renal transplantation. This decline in CTLp was initially nonspecific, as CTLp frequencies against third-party animals also declined (P<0.01). The decrease in CTLp was maintained in five of five animals tested 6 months after transplant. However, unresponsiveness was limited to the CTL arm of the immune response as antidonor IgG was detected in four of four animals tested, and the 5-day mixed lymphocyte response stimulation index and relative response were not significantly different before and after transplant. In long-term survivors (>150 days), an increase in anti-third-party CTLp was detected 1 month after grafting with third-party skin. No change was seen in the antidonor CTLp frequency after donor skin grafting, indicating that a specific defect in the antidonor CTL response had developed. CONCLUSIONS: These data suggest that FN18-CRM9 treatment of rhesus monkeys allows the development of specific down-regulation of antidonor CTL activity in renal allograft recipients.

Duke Scholars

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

May 15, 1997

Volume

63

Issue

9

Start / End Page

1339 / 1345

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes, Cytotoxic
  • T-Lymphocytes
  • Surgery
  • Skin Transplantation
  • Macaca mulatta
  • Lymphocyte Subsets
  • Lymphocyte Culture Test, Mixed
  • Kidney Transplantation
 

Citation

APA
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MLA
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Fechner, J. H., Vargo, D. J., Geissler, E. K., Graeb, C., Wang, J., Hanaway, M. J., … Knechtle, S. J. (1997). Split tolerance induced by immunotoxin in a rhesus kidney allograft model. Transplantation, 63(9), 1339–1345. https://doi.org/10.1097/00007890-199705150-00023
Fechner, J. H., D. J. Vargo, E. K. Geissler, C. Graeb, J. Wang, M. J. Hanaway, D. I. Watkins, M. Piekarczyk, D. M. Neville, and S. J. Knechtle. “Split tolerance induced by immunotoxin in a rhesus kidney allograft model.Transplantation 63, no. 9 (May 15, 1997): 1339–45. https://doi.org/10.1097/00007890-199705150-00023.
Fechner JH, Vargo DJ, Geissler EK, Graeb C, Wang J, Hanaway MJ, et al. Split tolerance induced by immunotoxin in a rhesus kidney allograft model. Transplantation. 1997 May 15;63(9):1339–45.
Fechner, J. H., et al. “Split tolerance induced by immunotoxin in a rhesus kidney allograft model.Transplantation, vol. 63, no. 9, May 1997, pp. 1339–45. Pubmed, doi:10.1097/00007890-199705150-00023.
Fechner JH, Vargo DJ, Geissler EK, Graeb C, Wang J, Hanaway MJ, Watkins DI, Piekarczyk M, Neville DM, Knechtle SJ. Split tolerance induced by immunotoxin in a rhesus kidney allograft model. Transplantation. 1997 May 15;63(9):1339–1345.

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

May 15, 1997

Volume

63

Issue

9

Start / End Page

1339 / 1345

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes, Cytotoxic
  • T-Lymphocytes
  • Surgery
  • Skin Transplantation
  • Macaca mulatta
  • Lymphocyte Subsets
  • Lymphocyte Culture Test, Mixed
  • Kidney Transplantation