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Increased proportion of FoxP3+ regulatory T cells in tumor infiltrating lymphocytes is associated with tumor recurrence and reduced survival in patients with glioblastoma.

Publication ,  Journal Article
Sayour, EJ; McLendon, P; McLendon, R; De Leon, G; Reynolds, R; Kresak, J; Sampson, JH; Mitchell, DA
Published in: Cancer Immunol Immunother
April 2015

Glioblastoma multiforme (GBM) is an aggressive malignancy associated with profound host immunosuppression mediated in part by FoxP3 expressing regulatory CD4+ T lymphocytes (Tregs) that down-regulate anti-tumor immunity. In order to assess whether FoxP3 was an independent driver differentially expressed in primary versus recurrent GBMs, we stained resected primary and recurrent GBM tumors for CD3, CD4, CD8 and FoxP3 expression using standard immunohistochemistry. Slides were scanned with a high-resolution scanner (ScanScope CS; Aperio), and image analysis software (Aperio ScanScope) was used to enumerate lymphocyte subpopulations allowing for high-throughput analysis and bypassing manual selection bias. As shown in previous studies, enumeration of individual lymphocyte populations did not correlate with clinical outcomes in patients with GBM. However, the CD4+ to regulatory FoxP3+ T cell ratio was diminished in recurrent disease, and increased CD3 and CD8+ to regulatory T cell ratios showed a positive correlation with survival outcomes in primary GBM. These results suggest that while absolute numbers of tumor infiltrating lymphocytes may not be informative for predicting clinical outcomes in patients with GBM, the effective balance of CD3, CD4 and CD8+ T cells to immunosuppressive FoxP3+ regulatory cells may influence clinical outcomes in this patient population.

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Published In

Cancer Immunol Immunother

DOI

EISSN

1432-0851

Publication Date

April 2015

Volume

64

Issue

4

Start / End Page

419 / 427

Location

Germany

Related Subject Headings

  • T-Lymphocytes, Regulatory
  • Survival Rate
  • Prognosis
  • Neoplasm Staging
  • Neoplasm Recurrence, Local
  • Middle Aged
  • Male
  • Lymphocytes, Tumor-Infiltrating
  • Immunology
  • Immunoenzyme Techniques
 

Citation

APA
Chicago
ICMJE
MLA
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Sayour, E. J., McLendon, P., McLendon, R., De Leon, G., Reynolds, R., Kresak, J., … Mitchell, D. A. (2015). Increased proportion of FoxP3+ regulatory T cells in tumor infiltrating lymphocytes is associated with tumor recurrence and reduced survival in patients with glioblastoma. Cancer Immunol Immunother, 64(4), 419–427. https://doi.org/10.1007/s00262-014-1651-7
Sayour, Elias J., Pat McLendon, Roger McLendon, Gabriel De Leon, Renee Reynolds, Jesse Kresak, John H. Sampson, and Duane A. Mitchell. “Increased proportion of FoxP3+ regulatory T cells in tumor infiltrating lymphocytes is associated with tumor recurrence and reduced survival in patients with glioblastoma.Cancer Immunol Immunother 64, no. 4 (April 2015): 419–27. https://doi.org/10.1007/s00262-014-1651-7.
Sayour EJ, McLendon P, McLendon R, De Leon G, Reynolds R, Kresak J, et al. Increased proportion of FoxP3+ regulatory T cells in tumor infiltrating lymphocytes is associated with tumor recurrence and reduced survival in patients with glioblastoma. Cancer Immunol Immunother. 2015 Apr;64(4):419–27.
Sayour, Elias J., et al. “Increased proportion of FoxP3+ regulatory T cells in tumor infiltrating lymphocytes is associated with tumor recurrence and reduced survival in patients with glioblastoma.Cancer Immunol Immunother, vol. 64, no. 4, Apr. 2015, pp. 419–27. Pubmed, doi:10.1007/s00262-014-1651-7.
Sayour EJ, McLendon P, McLendon R, De Leon G, Reynolds R, Kresak J, Sampson JH, Mitchell DA. Increased proportion of FoxP3+ regulatory T cells in tumor infiltrating lymphocytes is associated with tumor recurrence and reduced survival in patients with glioblastoma. Cancer Immunol Immunother. 2015 Apr;64(4):419–427.
Journal cover image

Published In

Cancer Immunol Immunother

DOI

EISSN

1432-0851

Publication Date

April 2015

Volume

64

Issue

4

Start / End Page

419 / 427

Location

Germany

Related Subject Headings

  • T-Lymphocytes, Regulatory
  • Survival Rate
  • Prognosis
  • Neoplasm Staging
  • Neoplasm Recurrence, Local
  • Middle Aged
  • Male
  • Lymphocytes, Tumor-Infiltrating
  • Immunology
  • Immunoenzyme Techniques