Pulse Field Gel Electrophoresis.
Publication
, Journal Article
Sharma-Kuinkel, BK; Rude, TH; Fowler, VG
Published in: Methods Mol Biol
2016
Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2016
Volume
1373
Start / End Page
117 / 130
Location
United States
Related Subject Headings
- Genotype
- Electrophoresis, Gel, Pulsed-Field
- Developmental Biology
- DNA, Bacterial
- DNA Restriction Enzymes
- DNA
- Chromosomes, Bacterial
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Sharma-Kuinkel, B. K., Rude, T. H., & Fowler, V. G. (2016). Pulse Field Gel Electrophoresis. Methods Mol Biol, 1373, 117–130. https://doi.org/10.1007/7651_2014_191
Sharma-Kuinkel, Batu K., Thomas H. Rude, and Vance G. Fowler. “Pulse Field Gel Electrophoresis.” Methods Mol Biol 1373 (2016): 117–30. https://doi.org/10.1007/7651_2014_191.
Sharma-Kuinkel BK, Rude TH, Fowler VG. Pulse Field Gel Electrophoresis. Methods Mol Biol. 2016;1373:117–30.
Sharma-Kuinkel, Batu K., et al. “Pulse Field Gel Electrophoresis.” Methods Mol Biol, vol. 1373, 2016, pp. 117–30. Pubmed, doi:10.1007/7651_2014_191.
Sharma-Kuinkel BK, Rude TH, Fowler VG. Pulse Field Gel Electrophoresis. Methods Mol Biol. 2016;1373:117–130.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2016
Volume
1373
Start / End Page
117 / 130
Location
United States
Related Subject Headings
- Genotype
- Electrophoresis, Gel, Pulsed-Field
- Developmental Biology
- DNA, Bacterial
- DNA Restriction Enzymes
- DNA
- Chromosomes, Bacterial
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology