A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Publication
, Journal Article
Polstein, LR; Gersbach, CA
Published in: Nature chemical biology
March 2015
Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
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Published In
Nature chemical biology
DOI
EISSN
1552-4469
ISSN
1552-4450
Publication Date
March 2015
Volume
11
Issue
3
Start / End Page
198 / 200
Related Subject Headings
- Transcriptional Activation
- Transcription, Genetic
- Streptococcus pyogenes
- RNA
- Protein Engineering
- Plasmids
- Optogenetics
- Light
- Humans
- Gene Expression Regulation
Citation
APA
Chicago
ICMJE
MLA
NLM
Polstein, L. R., & Gersbach, C. A. (2015). A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature Chemical Biology, 11(3), 198–200. https://doi.org/10.1038/nchembio.1753
Polstein, Lauren R., and Charles A. Gersbach. “A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.” Nature Chemical Biology 11, no. 3 (March 2015): 198–200. https://doi.org/10.1038/nchembio.1753.
Polstein LR, Gersbach CA. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature chemical biology. 2015 Mar;11(3):198–200.
Polstein, Lauren R., and Charles A. Gersbach. “A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.” Nature Chemical Biology, vol. 11, no. 3, Mar. 2015, pp. 198–200. Epmc, doi:10.1038/nchembio.1753.
Polstein LR, Gersbach CA. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature chemical biology. 2015 Mar;11(3):198–200.
Published In
Nature chemical biology
DOI
EISSN
1552-4469
ISSN
1552-4450
Publication Date
March 2015
Volume
11
Issue
3
Start / End Page
198 / 200
Related Subject Headings
- Transcriptional Activation
- Transcription, Genetic
- Streptococcus pyogenes
- RNA
- Protein Engineering
- Plasmids
- Optogenetics
- Light
- Humans
- Gene Expression Regulation