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Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In Vitro.

Publication ,  Journal Article
Peters, EB; Liu, B; Christoforou, N; West, JL; Truskey, GA
Published in: Annals of Biomedical Engineering
October 2015

Umbilical cord blood represents a promising cell source for pro-angiogenic therapies. The present study examined the potential of mononuclear cells (MNCs) from umbilical cord blood to support endothelial progenitor cell (EPC) microvessel formation. MNCs were isolated from the cord blood of 20 separate donors and selected for further characterization based upon their proliferation potential and morphological resemblance to human vascular pericytes (HVPs). MNCs were screened for their ability to support EPC network formation using an in vitro assay (Matrigel™) as well as a reductionist, coculture system consisting of no additional angiogenic cytokines beyond those present in serum. In less than 15% of the isolations, we identified a population of highly proliferative MNCs that phenotypically resembled HVPs as assessed by expression of PDGFR-β, NG2, α-SMA, and ephrin-B2. Within a Matrigel™ system, MNCs demonstrated pericyte-like function through colocalization to EPC networks and similar effects as HVPs upon total EPC tubule length (p = 0.95) and number of branch points (p = 0.93). In a reductionist coculture system, MNCs served as pro-angiogenic mural cells by supporting EPC network formation to a significantly greater extent than HVP cocultures, by day 14 of coculture, as evidenced through EPC total tubule length (p < 0.0001) and number of branch points (p < 0.0001). Our findings are significant as we demonstrate mural cell progenitors can be isolated from umbilical cord blood and develop culture conditions to support their use in microvascular tissue engineering applications.

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Published In

Annals of Biomedical Engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

October 2015

Volume

43

Issue

10

Start / End Page

2552 / 2568

Related Subject Headings

  • Stem Cells
  • Pericytes
  • Neovascularization, Physiologic
  • Humans
  • Gene Expression Regulation
  • Fetal Blood
  • Endothelial Cells
  • Cytokines
  • Biomedical Engineering
  • Antigens, Differentiation
 

Citation

APA
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ICMJE
MLA
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Peters, E. B., Liu, B., Christoforou, N., West, J. L., & Truskey, G. A. (2015). Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In Vitro. Annals of Biomedical Engineering, 43(10), 2552–2568. https://doi.org/10.1007/s10439-015-1301-z
Peters, Erica B., Betty Liu, Nicolas Christoforou, Jennifer L. West, and George A. Truskey. “Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In Vitro.Annals of Biomedical Engineering 43, no. 10 (October 2015): 2552–68. https://doi.org/10.1007/s10439-015-1301-z.
Peters EB, Liu B, Christoforou N, West JL, Truskey GA. Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In Vitro. Annals of Biomedical Engineering. 2015 Oct;43(10):2552–68.
Peters, Erica B., et al. “Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In Vitro.Annals of Biomedical Engineering, vol. 43, no. 10, Oct. 2015, pp. 2552–68. Epmc, doi:10.1007/s10439-015-1301-z.
Peters EB, Liu B, Christoforou N, West JL, Truskey GA. Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In Vitro. Annals of Biomedical Engineering. 2015 Oct;43(10):2552–2568.
Journal cover image

Published In

Annals of Biomedical Engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

October 2015

Volume

43

Issue

10

Start / End Page

2552 / 2568

Related Subject Headings

  • Stem Cells
  • Pericytes
  • Neovascularization, Physiologic
  • Humans
  • Gene Expression Regulation
  • Fetal Blood
  • Endothelial Cells
  • Cytokines
  • Biomedical Engineering
  • Antigens, Differentiation