Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

In vitro metabolism of the flame retardant triphenyl phosphate in chicken embryonic hepatocytes and the importance of the hydroxylation pathway

Publication ,  Journal Article
Su, G; Letcher, RJ; Crump, D; Gooden, DM; Stapleton, HM
Published in: Environmental Science and Technology Letters
April 14, 2015

We report for the first time either in vitro or in vivo the phase I hydroxylation and phase II conjugation metabolic pathways of an organophosphate flame retardant, triphenyl phosphate (TPHP), in addition to diphenyl phosphate (DPHP) metabolite formation. Using a chicken embryonic hepatocyte (CEH) assay, TPHP was phase I metabolized to p- and m-hydroxy-TPHP metabolites, which were largely present in the assay medium and cells as phase II conjugates with glucuronic acid. After treatment with β-glucuronidase, deconjugated p-OH-TPHP was present in both the medium and cells at increasing concentrations of 0.073 ± 0.003, 1.95 ± 0.03, and 2.10 ± 0.09 nmol/well at CEH incubation time points of 0, 12, and 36 h, respectively. Similarly, after β-glucuronidase treatment, there were increasing m-OH-TPHP concentrations of 0.0050 ± 0.0005, 0.18 ± 0.01, and 0.18 ± 0.01 nmol/well. p-OH-TPHP at 36 h accounted for 60% of the initial TPHP treatment concentration, which was 3.5- or 12-fold greater than that of the DPHP or m-OH-TPHP metabolites, respectively. Overall, in TPHP-exposed organisms, this study demonstrates the importance of phase I and II metabolic processes in the biological fate of TPHP.

Duke Scholars

Published In

Environmental Science and Technology Letters

DOI

EISSN

2328-8930

Publication Date

April 14, 2015

Volume

2

Issue

4

Start / End Page

100 / 104

Related Subject Headings

  • 4105 Pollution and contamination
  • 4004 Chemical engineering
  • 1002 Environmental Biotechnology
  • 0907 Environmental Engineering
  • 0502 Environmental Science and Management
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Su, G., Letcher, R. J., Crump, D., Gooden, D. M., & Stapleton, H. M. (2015). In vitro metabolism of the flame retardant triphenyl phosphate in chicken embryonic hepatocytes and the importance of the hydroxylation pathway. Environmental Science and Technology Letters, 2(4), 100–104. https://doi.org/10.1021/acs.estlett.5b00041
Su, G., R. J. Letcher, D. Crump, D. M. Gooden, and H. M. Stapleton. “In vitro metabolism of the flame retardant triphenyl phosphate in chicken embryonic hepatocytes and the importance of the hydroxylation pathway.” Environmental Science and Technology Letters 2, no. 4 (April 14, 2015): 100–104. https://doi.org/10.1021/acs.estlett.5b00041.
Su G, Letcher RJ, Crump D, Gooden DM, Stapleton HM. In vitro metabolism of the flame retardant triphenyl phosphate in chicken embryonic hepatocytes and the importance of the hydroxylation pathway. Environmental Science and Technology Letters. 2015 Apr 14;2(4):100–4.
Su, G., et al. “In vitro metabolism of the flame retardant triphenyl phosphate in chicken embryonic hepatocytes and the importance of the hydroxylation pathway.” Environmental Science and Technology Letters, vol. 2, no. 4, Apr. 2015, pp. 100–04. Scopus, doi:10.1021/acs.estlett.5b00041.
Su G, Letcher RJ, Crump D, Gooden DM, Stapleton HM. In vitro metabolism of the flame retardant triphenyl phosphate in chicken embryonic hepatocytes and the importance of the hydroxylation pathway. Environmental Science and Technology Letters. 2015 Apr 14;2(4):100–104.
Journal cover image

Published In

Environmental Science and Technology Letters

DOI

EISSN

2328-8930

Publication Date

April 14, 2015

Volume

2

Issue

4

Start / End Page

100 / 104

Related Subject Headings

  • 4105 Pollution and contamination
  • 4004 Chemical engineering
  • 1002 Environmental Biotechnology
  • 0907 Environmental Engineering
  • 0502 Environmental Science and Management