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Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants.

Publication ,  Journal Article
Scanlan, LD; Loguinov, AV; Teng, Q; Antczak, P; Dailey, KP; Nowinski, DT; Kornbluh, J; Lin, XX; Lachenauer, E; Arai, A; Douglas, NK; Vulpe, CD ...
Published in: Environmental science & technology
June 2015

The use of chemical flame-retardants (FR) in consumer products has steadily increased over the last 30 years. Toxicity data exist for legacy FRs such as pentabromodiphenyl ether (pentaBDE), but less is known about effects of new formulations. To address this issue, the toxicity of seven FR chemicals and formulations was assessed on the freshwater crustacean Daphnia magna. Acute 48-h nominal LC50 values for penta- and octabromodiphenyl ether (pentaBDE, octaBDE), Firemaster 550 (FM550), Firemaster BZ-54 (BZ54), bis(2-ethylhexyl) tetrabromophthalate (BEH-TEBP), triphenyl phosphate (TPhP), and nonbrominated BEH-TEBP analog bis(2-ethylhexyl) phthalate (BEHP) ranged from 0.058 mg/L (pentaBDE) to 3.96 mg/L (octaBDE). mRNA expression, (1)H NMR-based metabolomic and lipidomic profiling at 1/10 LC50 revealed distinct patterns of molecular response for each exposure, suggesting pentaPBDE affects transcription and translation, octaBDE and BEH-TEBP affect glycosphingolipid biosynthesis and BZ54 affects Wnt and Hedgehog signal pathways as well as glycosaminoglycan degradation. Brominated components of FM550 (i.e., BZ54) were significantly higher in Daphnia after 48 h following 1/10 LC50 exposure. FM550 elicited significant mRNA changes at five concentrations across a range from 1/10(6) LC50 to 1/2 LC50. Analyses suggest FM550 impairs nutrient utilization or uptake in Daphnia.

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Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

June 2015

Volume

49

Issue

12

Start / End Page

7400 / 7410

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • Proton Magnetic Resonance Spectroscopy
  • Metabolomics
  • Metabolome
  • Lipid Metabolism
  • Gene Expression Profiling
  • Flame Retardants
  • Environmental Sciences
  • Environmental Exposure
 

Citation

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Scanlan, L. D., Loguinov, A. V., Teng, Q., Antczak, P., Dailey, K. P., Nowinski, D. T., … Vulpe, C. D. (2015). Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants. Environmental Science & Technology, 49(12), 7400–7410. https://doi.org/10.1021/acs.est.5b00977
Scanlan, Leona D., Alexandre V. Loguinov, Quincy Teng, Philipp Antczak, Kathleen P. Dailey, Daniel T. Nowinski, Jonah Kornbluh, et al. “Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants.Environmental Science & Technology 49, no. 12 (June 2015): 7400–7410. https://doi.org/10.1021/acs.est.5b00977.
Scanlan LD, Loguinov AV, Teng Q, Antczak P, Dailey KP, Nowinski DT, et al. Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants. Environmental science & technology. 2015 Jun;49(12):7400–10.
Scanlan, Leona D., et al. “Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants.Environmental Science & Technology, vol. 49, no. 12, June 2015, pp. 7400–10. Epmc, doi:10.1021/acs.est.5b00977.
Scanlan LD, Loguinov AV, Teng Q, Antczak P, Dailey KP, Nowinski DT, Kornbluh J, Lin XX, Lachenauer E, Arai A, Douglas NK, Falciani F, Stapleton HM, Vulpe CD. Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants. Environmental science & technology. 2015 Jun;49(12):7400–7410.
Journal cover image

Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

June 2015

Volume

49

Issue

12

Start / End Page

7400 / 7410

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • Proton Magnetic Resonance Spectroscopy
  • Metabolomics
  • Metabolome
  • Lipid Metabolism
  • Gene Expression Profiling
  • Flame Retardants
  • Environmental Sciences
  • Environmental Exposure