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Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.

Publication ,  Journal Article
Jamiolkowski, RM; Kang, SD; Rodriguez, AK; Haseltine, JM; Galinat, LJ; Jantzen, AE; Carlon, TA; Darrabie, MD; Arciniegas, AJ; Mantilla, JG ...
Published in: Regenerative medicine
May 2015

Peripheral blood-derived endothelial cells (pBD-ECs) are an attractive tool for cell therapies and tissue engineering, but have been limited by their low isolation yield. We increase pBD-EC yield via administration of the chemokine receptor type 4 antagonist AMD3100, as well as via a diluted whole blood incubation (DWBI).Porcine pBD-ECs were isolated using AMD3100 and DWBI and tested for EC markers, acetylated LDL uptake, growth kinetics, metabolic activity, flow-mediated nitric oxide production and seeded onto titanium tubes implanted into vessels of pigs.DWBI increased the yield of porcine pBD-ECs 6.6-fold, and AMD3100 increased the yield 4.5-fold. AMD3100-mobilized ECs were phenotypically indistinguishable from nonmobilized ECs. In porcine implants, the cells expressed endothelial nitric oxide synthase, reduced thrombin-antithrombin complex systemically and prevented thrombosis.Administration of AMD3100 and the DWBI method both increase pBD-EC yield.

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Published In

Regenerative medicine

DOI

EISSN

1746-076X

ISSN

1746-0751

Publication Date

May 2015

Volume

10

Issue

4

Start / End Page

447 / 460

Related Subject Headings

  • Vena Cava, Inferior
  • Transplantation, Autologous
  • Tissue Engineering
  • Sus scrofa
  • Stress, Mechanical
  • Rheology
  • Models, Animal
  • Heterocyclic Compounds
  • Flow Cytometry
  • Endothelial Cells
 

Citation

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Jamiolkowski, R. M., Kang, S. D., Rodriguez, A. K., Haseltine, J. M., Galinat, L. J., Jantzen, A. E., … Achneck, H. E. (2015). Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering. Regenerative Medicine, 10(4), 447–460. https://doi.org/10.2217/rme.15.2
Jamiolkowski, Ryan M., Sa Do Kang, AnnMarie K. Rodriguez, Justin M. Haseltine, Lauren J. Galinat, Alexandra E. Jantzen, Tim A. Carlon, et al. “Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.Regenerative Medicine 10, no. 4 (May 2015): 447–60. https://doi.org/10.2217/rme.15.2.
Jamiolkowski RM, Kang SD, Rodriguez AK, Haseltine JM, Galinat LJ, Jantzen AE, et al. Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering. Regenerative medicine. 2015 May;10(4):447–60.
Jamiolkowski, Ryan M., et al. “Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.Regenerative Medicine, vol. 10, no. 4, May 2015, pp. 447–60. Epmc, doi:10.2217/rme.15.2.
Jamiolkowski RM, Kang SD, Rodriguez AK, Haseltine JM, Galinat LJ, Jantzen AE, Carlon TA, Darrabie MD, Arciniegas AJ, Mantilla JG, Haley NR, Noviani M, Allen JD, Stabler TV, Frederiksen JW, Alzate O, Keil LG, Liu S, Lin F-H, Truskey GA, Achneck HE. Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering. Regenerative medicine. 2015 May;10(4):447–460.
Journal cover image

Published In

Regenerative medicine

DOI

EISSN

1746-076X

ISSN

1746-0751

Publication Date

May 2015

Volume

10

Issue

4

Start / End Page

447 / 460

Related Subject Headings

  • Vena Cava, Inferior
  • Transplantation, Autologous
  • Tissue Engineering
  • Sus scrofa
  • Stress, Mechanical
  • Rheology
  • Models, Animal
  • Heterocyclic Compounds
  • Flow Cytometry
  • Endothelial Cells