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Sequence-specific B-DNA flexibility modulates Z-DNA formation.

Publication ,  Journal Article
Bothe, JR; Lowenhaupt, K; Al-Hashimi, HM
Published in: J Am Chem Soc
February 23, 2011

Conversion of right-handed B-DNA into left-handed Z-DNA is one of the largest structural transitions in biology that plays fundamental roles in gene expression and regulation. Z-DNA segments must form within genomes surrounded by a sea of B-DNA and require creation of energetically costly B/Z junctions. Here, we show using a combination of natural abundance NMR R(1ρ) carbon relaxation measurements and CD spectroscopy that sequence-specific B-DNA flexibility modulates the thermodynamic propensity to form Z-DNA and the location of B/Z junctions. We observe sequence-specific flexibility in B-DNA spanning fast (ps-ns) and slow (μs-ms) time scales localized at the site of B/Z junction formation. Further, our studies show that CG-repeats play an active role tuning this intrinsic B-DNA flexibility. Taken together, our results suggest that sequence-specific B-DNA flexibility may provide a mechanism for defining the length and location of Z-DNA in genomes.

Duke Scholars

Published In

J Am Chem Soc

DOI

EISSN

1520-5126

Publication Date

February 23, 2011

Volume

133

Issue

7

Start / End Page

2016 / 2018

Location

United States

Related Subject Headings

  • Molecular Sequence Data
  • Magnetic Resonance Spectroscopy
  • General Chemistry
  • DNA, Z-Form
  • DNA
  • Crystallography, X-Ray
  • Circular Dichroism
  • Base Sequence
  • 03 Chemical Sciences
 

Citation

APA
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ICMJE
MLA
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Bothe, J. R., Lowenhaupt, K., & Al-Hashimi, H. M. (2011). Sequence-specific B-DNA flexibility modulates Z-DNA formation. J Am Chem Soc, 133(7), 2016–2018. https://doi.org/10.1021/ja1073068
Bothe, Jameson R., Ky Lowenhaupt, and Hashim M. Al-Hashimi. “Sequence-specific B-DNA flexibility modulates Z-DNA formation.J Am Chem Soc 133, no. 7 (February 23, 2011): 2016–18. https://doi.org/10.1021/ja1073068.
Bothe JR, Lowenhaupt K, Al-Hashimi HM. Sequence-specific B-DNA flexibility modulates Z-DNA formation. J Am Chem Soc. 2011 Feb 23;133(7):2016–8.
Bothe, Jameson R., et al. “Sequence-specific B-DNA flexibility modulates Z-DNA formation.J Am Chem Soc, vol. 133, no. 7, Feb. 2011, pp. 2016–18. Pubmed, doi:10.1021/ja1073068.
Bothe JR, Lowenhaupt K, Al-Hashimi HM. Sequence-specific B-DNA flexibility modulates Z-DNA formation. J Am Chem Soc. 2011 Feb 23;133(7):2016–2018.
Journal cover image

Published In

J Am Chem Soc

DOI

EISSN

1520-5126

Publication Date

February 23, 2011

Volume

133

Issue

7

Start / End Page

2016 / 2018

Location

United States

Related Subject Headings

  • Molecular Sequence Data
  • Magnetic Resonance Spectroscopy
  • General Chemistry
  • DNA, Z-Form
  • DNA
  • Crystallography, X-Ray
  • Circular Dichroism
  • Base Sequence
  • 03 Chemical Sciences