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Rapid determination of protein folds using residual dipolar couplings.

Publication ,  Journal Article
Fowler, CA; Tian, F; Al-Hashimi, HM; Prestegard, JH
Published in: J Mol Biol
December 1, 2000

Over the next few years, various genome projects will sequence many new genes and yield many new gene products. Many of these products will have no known function and little, if any, sequence homology to existing proteins. There is reason to believe that a rapid determination of a protein fold, even at low resolution, can aid in the identification of function and expedite the determination of structure at higher resolution. Recently devised NMR methods of measuring residual dipolar couplings provide one route to the determination of a fold. They do this by allowing the alignment of previously identified secondary structural elements with respect to each other. When combined with constraints involving loops connecting elements or other short-range experimental distance information, a fold is produced. We illustrate this approach to protein fold determination on (15)N-labeled Eschericia coli acyl carrier protein using a limited set of (15)N-(1)H and (1)H-(1)H dipolar couplings. We also illustrate an approach using a more extended set of heteronuclear couplings on a related protein, (13)C, (15)N-labeled NodF protein from Rhizobium leguminosarum.

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Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

December 1, 2000

Volume

304

Issue

3

Start / End Page

447 / 460

Location

Netherlands

Related Subject Headings

  • Software
  • Rhizobium leguminosarum
  • Protein Structure, Secondary
  • Protein Folding
  • Nitrogen Isotopes
  • Models, Molecular
  • Magnetic Resonance Spectroscopy
  • Escherichia coli
  • Deuterium
  • Carbon Isotopes
 

Citation

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Fowler, C. A., Tian, F., Al-Hashimi, H. M., & Prestegard, J. H. (2000). Rapid determination of protein folds using residual dipolar couplings. J Mol Biol, 304(3), 447–460. https://doi.org/10.1006/jmbi.2000.4199
Fowler, C. A., F. Tian, H. M. Al-Hashimi, and J. H. Prestegard. “Rapid determination of protein folds using residual dipolar couplings.J Mol Biol 304, no. 3 (December 1, 2000): 447–60. https://doi.org/10.1006/jmbi.2000.4199.
Fowler CA, Tian F, Al-Hashimi HM, Prestegard JH. Rapid determination of protein folds using residual dipolar couplings. J Mol Biol. 2000 Dec 1;304(3):447–60.
Fowler, C. A., et al. “Rapid determination of protein folds using residual dipolar couplings.J Mol Biol, vol. 304, no. 3, Dec. 2000, pp. 447–60. Pubmed, doi:10.1006/jmbi.2000.4199.
Fowler CA, Tian F, Al-Hashimi HM, Prestegard JH. Rapid determination of protein folds using residual dipolar couplings. J Mol Biol. 2000 Dec 1;304(3):447–460.
Journal cover image

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

December 1, 2000

Volume

304

Issue

3

Start / End Page

447 / 460

Location

Netherlands

Related Subject Headings

  • Software
  • Rhizobium leguminosarum
  • Protein Structure, Secondary
  • Protein Folding
  • Nitrogen Isotopes
  • Models, Molecular
  • Magnetic Resonance Spectroscopy
  • Escherichia coli
  • Deuterium
  • Carbon Isotopes