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Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains.

Publication ,  Journal Article
Yang, H; Shi, J; Zhang, G; Yang, J; Delaloye, K; Cui, J
Published in: Nat Struct Mol Biol
November 2008

The voltage-sensor domain (VSD) and the ligand sensor (cytoplasmic domain) of BK channels synergistically control channel activities, thereby integrating electrical and chemical signals for cell function. Studies show that intracellular Mg2+ mediates the interaction between these sensory domains to activate the channel through an electrostatic interaction with the VSD. Here we report that Mg2+ binds to a site that consists of amino acid side chains from both the VSD (Asp99 and Asn172) and the cytoplasmic domain (Glu374 and Glu399). For each Mg2+ binding site, the residues in the VSD and those in the cytoplasmic domain come from neighboring subunits. These results suggest that the VSD and the cytoplasmic domains from different subunits may interact during channel gating, and the packing of VSD or the RCK1 domain to the pore in BK channels differ from that in Kv1.2 or MthK channels.

Duke Scholars

Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

November 2008

Volume

15

Issue

11

Start / End Page

1152 / 1159

Location

United States

Related Subject Headings

  • Sequence Alignment
  • Protein Subunits
  • Protein Structure, Tertiary
  • Protein Structure, Quaternary
  • Potassium Channels
  • Patch-Clamp Techniques
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Magnesium
 

Citation

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Yang, H., Shi, J., Zhang, G., Yang, J., Delaloye, K., & Cui, J. (2008). Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains. Nat Struct Mol Biol, 15(11), 1152–1159. https://doi.org/10.1038/nsmb.1507
Yang, Huanghe, Jingyi Shi, Guohui Zhang, Junqiu Yang, Kelli Delaloye, and Jianmin Cui. “Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains.Nat Struct Mol Biol 15, no. 11 (November 2008): 1152–59. https://doi.org/10.1038/nsmb.1507.
Yang H, Shi J, Zhang G, Yang J, Delaloye K, Cui J. Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains. Nat Struct Mol Biol. 2008 Nov;15(11):1152–9.
Yang, Huanghe, et al. “Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains.Nat Struct Mol Biol, vol. 15, no. 11, Nov. 2008, pp. 1152–59. Pubmed, doi:10.1038/nsmb.1507.
Yang H, Shi J, Zhang G, Yang J, Delaloye K, Cui J. Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains. Nat Struct Mol Biol. 2008 Nov;15(11):1152–1159.

Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

November 2008

Volume

15

Issue

11

Start / End Page

1152 / 1159

Location

United States

Related Subject Headings

  • Sequence Alignment
  • Protein Subunits
  • Protein Structure, Tertiary
  • Protein Structure, Quaternary
  • Potassium Channels
  • Patch-Clamp Techniques
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Magnesium