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A ubiquitin E2 variant protein acts in axon termination and synaptogenesis in Caenorhabditis elegans.

Publication ,  Journal Article
Trujillo, G; Nakata, K; Yan, D; Maruyama, IN; Jin, Y
Published in: Genetics
September 2010

In the developing nervous system, cohorts of events regulate the precise patterning of axons and formation of synapses between presynaptic neurons and their targets. The conserved PHR proteins play important roles in many aspects of axon and synapse development from C. elegans to mammals. The PHR proteins act as E3 ubiquitin ligases for the dual-leucine-zipper-bearing MAP kinase kinase kinase (DLK MAPKKK) to regulate the signal transduction cascade. In C. elegans, loss-of-function of the PHR protein RPM-1 (Regulator of Presynaptic Morphology-1) results in fewer synapses, disorganized presynaptic architecture, and axon overextension. Inactivation of the DLK-1 pathway suppresses these defects. By characterizing additional genetic suppressors of rpm-1, we present here a new member of the DLK-1 pathway, UEV-3, an E2 ubiquitin-conjugating enzyme variant. We show that uev-3 acts cell autonomously in neurons, despite its ubiquitous expression. Our genetic epistasis analysis supports a conclusion that uev-3 acts downstream of the MAPKK mkk-4 and upstream of the MAPKAPK mak-2. UEV-3 can interact with the p38 MAPK PMK-3. We postulate that UEV-3 may provide additional specificity in the DLK-1 pathway by contributing to activation of PMK-3 or limiting the substrates accessible to PMK-3.

Duke Scholars

Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

September 2010

Volume

186

Issue

1

Start / End Page

135 / 145

Location

United States

Related Subject Headings

  • Ubiquitin-Conjugating Enzymes
  • Substrate Specificity
  • Presynaptic Terminals
  • Motor Neurons
  • Molecular Sequence Data
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • MAP Kinase Signaling System
  • MAP Kinase Kinase Kinases
  • Guanine Nucleotide Exchange Factors
 

Citation

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ICMJE
MLA
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Trujillo, G., Nakata, K., Yan, D., Maruyama, I. N., & Jin, Y. (2010). A ubiquitin E2 variant protein acts in axon termination and synaptogenesis in Caenorhabditis elegans. Genetics, 186(1), 135–145. https://doi.org/10.1534/genetics.110.117341
Trujillo, Gloriana, Katsunori Nakata, Dong Yan, Ichi N. Maruyama, and Yishi Jin. “A ubiquitin E2 variant protein acts in axon termination and synaptogenesis in Caenorhabditis elegans.Genetics 186, no. 1 (September 2010): 135–45. https://doi.org/10.1534/genetics.110.117341.
Trujillo G, Nakata K, Yan D, Maruyama IN, Jin Y. A ubiquitin E2 variant protein acts in axon termination and synaptogenesis in Caenorhabditis elegans. Genetics. 2010 Sep;186(1):135–45.
Trujillo, Gloriana, et al. “A ubiquitin E2 variant protein acts in axon termination and synaptogenesis in Caenorhabditis elegans.Genetics, vol. 186, no. 1, Sept. 2010, pp. 135–45. Pubmed, doi:10.1534/genetics.110.117341.
Trujillo G, Nakata K, Yan D, Maruyama IN, Jin Y. A ubiquitin E2 variant protein acts in axon termination and synaptogenesis in Caenorhabditis elegans. Genetics. 2010 Sep;186(1):135–145.

Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

September 2010

Volume

186

Issue

1

Start / End Page

135 / 145

Location

United States

Related Subject Headings

  • Ubiquitin-Conjugating Enzymes
  • Substrate Specificity
  • Presynaptic Terminals
  • Motor Neurons
  • Molecular Sequence Data
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • MAP Kinase Signaling System
  • MAP Kinase Kinase Kinases
  • Guanine Nucleotide Exchange Factors