Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.
Publication
, Journal Article
Zhang, G; Cai, F; de Rivera, IL; Zhou, Z; Zhang, J; Nkengasong, J; Gao, F; Yang, C
Published in: J Clin Microbiol
January 2016
A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing.
Duke Scholars
Published In
J Clin Microbiol
DOI
EISSN
1098-660X
Publication Date
January 2016
Volume
54
Issue
1
Start / End Page
220 / 222
Location
United States
Related Subject Headings
- Specimen Handling
- Mutation, Missense
- Microbiology
- Microbial Sensitivity Tests
- Humans
- HIV-1
- HIV Infections
- Genotyping Techniques
- Drug Resistance, Viral
- Desiccation
Citation
APA
Chicago
ICMJE
MLA
NLM
Zhang, G., Cai, F., de Rivera, I. L., Zhou, Z., Zhang, J., Nkengasong, J., … Yang, C. (2016). Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay. J Clin Microbiol, 54(1), 220–222. https://doi.org/10.1128/JCM.02833-15
Zhang, Guoqing, Fangping Cai, Ivette Lorenzana de Rivera, Zhiyong Zhou, Jing Zhang, John Nkengasong, Feng Gao, and Chunfu Yang. “Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.” J Clin Microbiol 54, no. 1 (January 2016): 220–22. https://doi.org/10.1128/JCM.02833-15.
Zhang G, Cai F, de Rivera IL, Zhou Z, Zhang J, Nkengasong J, et al. Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay. J Clin Microbiol. 2016 Jan;54(1):220–2.
Zhang, Guoqing, et al. “Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.” J Clin Microbiol, vol. 54, no. 1, Jan. 2016, pp. 220–22. Pubmed, doi:10.1128/JCM.02833-15.
Zhang G, Cai F, de Rivera IL, Zhou Z, Zhang J, Nkengasong J, Gao F, Yang C. Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay. J Clin Microbiol. 2016 Jan;54(1):220–222.
Published In
J Clin Microbiol
DOI
EISSN
1098-660X
Publication Date
January 2016
Volume
54
Issue
1
Start / End Page
220 / 222
Location
United States
Related Subject Headings
- Specimen Handling
- Mutation, Missense
- Microbiology
- Microbial Sensitivity Tests
- Humans
- HIV-1
- HIV Infections
- Genotyping Techniques
- Drug Resistance, Viral
- Desiccation