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Post-Thaw Colony Forming Unit (CFU) Counts and Yield Are the Most Important Predictors of Engraftment and Survival Following Unrelated Donor Cord Blood Transplantation (CBT): A COBLT Study Report.

Publication ,  Conference
Wall, DA; Wagner, EL; Carter, SL; Mendizabal, AM; Cairo, MS; Kernan, NA; Laughlin, M; Kurtzberg, J
Published in: Blood
November 16, 2005

Total nucleated cell count (TNC), CD34 and CFU content of cryopreserved cord blood units (CBUs) are measurements of hematopoietic potential previously demonstrated to be predictive of engraftment and/or survival post-transplant. Pre-cryopreservation measurements do not capture downstream events in cord blood banking that could impact CBT outcome: freezing, long-term storage, shipment to the transplant center and thawing. We hypothesized post-thaw enumeration of these measures would be more predictive of outcome. These tests were routinely performed at two of the COBLT transplant centers, at the time of CBU thaw. We examined the correlation of these laboratory measures pre-cryopreservation and post-thaw with engraftment and survival of 153 patients with leukemia and metabolic disorders undergoing myeloablative transplantation. The median age of recipients was 4.4 years (range 0.1 – 17.9) and weight 19.1 kg (range 3.9 – 118.4).Pre-cryopreservation Thaw Yield Median (range) Median (range) Median (range) TNC/kg x 107/kg 6.9 (1.5 – 50.4) 5.7 (1.1 – 31.6) 80.9% (43.0 – 98.9) CD34/kg x 105/kg 2.3 (0.1 – 20.1) 2.0 (0.2 – 15.5) 72.6% (16.1 – 99.6) CFU/kg x 104/kg 20.2 (1.7 – 169.7) 3.0 (0.0 – 31.1) 17.7% (0.0 – 82.7) Trypan blue viability 99 (91 – 100) 98 (83 – 100) —Overall 114/153 engrafted by day +42 with a median day to engraftment of 29 days. In multivariate analysis, post-thaw CFU count and yield were the highest correlates with engraftment (p<0.0001 and p<0.02, respectively) and survival (p=0.05 and p=0.02, respectively). If the CFU yield was 50–100% of pre-cryopreservation CFU then the probability of engraftment by day +42 was 93%. However, engraftment by day +42 fell to 63% when the yield was <5% (16% of CBUs). Survival at 2 years was poorer if CFU yield was less than 5% compared to >5% (38% vs. 65%, p =0.05). Importantly, post-thaw yield of TNC and trypan blue viability did not correlate with engraftment. Total hematopoietic content of units (TNC, CD34, and CFU) was strongly predictive of engraftment. However, independently, low CFU counts and poor yield of CFU at time of CBU thaw is a harbinger of poor/nonengraftment and is evidence of deterioration of the product between time of processing and infusion. Low CFU count or poor yield of CFU post-thaw may be used clinically to predict engraftment in patients undergoing CBT. These findings illustrate the need for the development of product release testing criteria for CBUs and should be a priority for the cord blood community.

Duke Scholars

Published In

Blood

DOI

EISSN

1528-0020

ISSN

0006-4971

Publication Date

November 16, 2005

Volume

106

Issue

11

Start / End Page

2046 / 2046

Publisher

American Society of Hematology

Related Subject Headings

  • Immunology
  • 1114 Paediatrics and Reproductive Medicine
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wall, D. A., Wagner, E. L., Carter, S. L., Mendizabal, A. M., Cairo, M. S., Kernan, N. A., … Kurtzberg, J. (2005). Post-Thaw Colony Forming Unit (CFU) Counts and Yield Are the Most Important Predictors of Engraftment and Survival Following Unrelated Donor Cord Blood Transplantation (CBT): A COBLT Study Report. In Blood (Vol. 106, pp. 2046–2046). American Society of Hematology. https://doi.org/10.1182/blood.v106.11.2046.2046
Wall, Donna A., Elizabeth L. Wagner, Shelly L. Carter, Adam M. Mendizabal, Mitchell S. Cairo, Nancy A. Kernan, Mary Laughlin, and Joanne Kurtzberg. “Post-Thaw Colony Forming Unit (CFU) Counts and Yield Are the Most Important Predictors of Engraftment and Survival Following Unrelated Donor Cord Blood Transplantation (CBT): A COBLT Study Report.” In Blood, 106:2046–2046. American Society of Hematology, 2005. https://doi.org/10.1182/blood.v106.11.2046.2046.
Wall DA, Wagner EL, Carter SL, Mendizabal AM, Cairo MS, Kernan NA, et al. Post-Thaw Colony Forming Unit (CFU) Counts and Yield Are the Most Important Predictors of Engraftment and Survival Following Unrelated Donor Cord Blood Transplantation (CBT): A COBLT Study Report. In: Blood. American Society of Hematology; 2005. p. 2046–2046.
Wall, Donna A., et al. “Post-Thaw Colony Forming Unit (CFU) Counts and Yield Are the Most Important Predictors of Engraftment and Survival Following Unrelated Donor Cord Blood Transplantation (CBT): A COBLT Study Report.Blood, vol. 106, no. 11, American Society of Hematology, 2005, pp. 2046–2046. Crossref, doi:10.1182/blood.v106.11.2046.2046.
Wall DA, Wagner EL, Carter SL, Mendizabal AM, Cairo MS, Kernan NA, Laughlin M, Kurtzberg J. Post-Thaw Colony Forming Unit (CFU) Counts and Yield Are the Most Important Predictors of Engraftment and Survival Following Unrelated Donor Cord Blood Transplantation (CBT): A COBLT Study Report. Blood. American Society of Hematology; 2005. p. 2046–2046.

Published In

Blood

DOI

EISSN

1528-0020

ISSN

0006-4971

Publication Date

November 16, 2005

Volume

106

Issue

11

Start / End Page

2046 / 2046

Publisher

American Society of Hematology

Related Subject Headings

  • Immunology
  • 1114 Paediatrics and Reproductive Medicine
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology