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Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells.

Publication ,  Journal Article
Shi, Q; Le, X; Wang, B; Abbruzzese, JL; Xiong, Q; He, Y; Xie, K
Published in: Oncogene
June 21, 2001
Published version (DOI) Link to item

The influence of acidosis on the expression of the vascular endothelial growth factor (VEGF) gene was determined. FG human pancreatic adenocarcinoma cells were incubated for various time periods in media at a physiologically relevant pH level (6.7-7.4). The expression of VEGF mRNA and protein secretion was inversely correlated with pH in a pH- and time-dependent manner. Transient acidosis also activated the VEGF promoter/enhancer luciferase reporter, which was consistent with an increased VEGF gene transcription rate and VEGF mRNA half-life. These data indicated that acidosis transcriptionally and posttranscriptionally regulates VEGF expression, suggesting that an acidic tumor microenvironment contributes to tumor angiogenesis and progression.

Duke Scholars

James Abbruzzese
Author James Abbruzzese Medicine, Medical Oncology
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Published In

Oncogene

DOI

10.1038/sj.onc.1204500

ISSN

0950-9232

Publication Date

June 21, 2001

Volume

20

Issue

28

Start / End Page

3751 / 3756

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Oncology & Carcinogenesis
  • Male
  • Lymphokines
  • Interleukin-8
  • Hydrogen-Ion Concentration
  • Humans
 

Citation

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Shi, Q., Le, X., Wang, B., Abbruzzese, J. L., Xiong, Q., He, Y., & Xie, K. (2001). Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells. Oncogene, 20(28), 3751–3756. https://doi.org/10.1038/sj.onc.1204500
Shi, Q., X. Le, B. Wang, J. L. Abbruzzese, Q. Xiong, Y. He, and K. Xie. “Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells.” Oncogene 20, no. 28 (June 21, 2001): 3751–56. https://doi.org/10.1038/sj.onc.1204500.
Shi Q, Le X, Wang B, Abbruzzese JL, Xiong Q, He Y, et al. Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells. Oncogene. 2001 Jun 21;20(28):3751–6.
Shi, Q., et al. “Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells.” Oncogene, vol. 20, no. 28, June 2001, pp. 3751–56. Pubmed, doi:10.1038/sj.onc.1204500.
Shi Q, Le X, Wang B, Abbruzzese JL, Xiong Q, He Y, Xie K. Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells. Oncogene. 2001 Jun 21;20(28):3751–3756.

Published In

Oncogene

DOI

10.1038/sj.onc.1204500

ISSN

0950-9232

Publication Date

June 21, 2001

Volume

20

Issue

28

Start / End Page

3751 / 3756

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Oncology & Carcinogenesis
  • Male
  • Lymphokines
  • Interleukin-8
  • Hydrogen-Ion Concentration
  • Humans