Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF.
Publication
, Journal Article
Person, MD; Shen, J; Traner, A; Hensley, SC; Lo, H-H; Abbruzzese, JL; Li, D
Published in: J Biomol Tech
April 2006
We previously reported a protein expression profiling experiment conducted on human pancreatic tissues using 2D gel electrophoresis and mass spectrometry. Here, 18 spots that were identified in the gel at molecular weights more than 10 kDa lower than database values are characterized. The matrix-assisted laser desorption/ionization mass spectrometry coverage is sufficient to identify the protein region present in each spot. Most of the fragments correspond to processed chains and known structural or functional domains, which may result from limited proteolysis.
Duke Scholars
Published In
J Biomol Tech
ISSN
1524-0215
Publication Date
April 2006
Volume
17
Issue
2
Start / End Page
145 / 156
Location
United States
Related Subject Headings
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Proteomics
- Proteins
- Protein Structure, Tertiary
- Protein Conformation
- Peptides
- Peptide Hydrolases
- Pancreas
- Molecular Sequence Data
- Molecular Chaperones
Citation
APA
Chicago
ICMJE
MLA
NLM
Person, M. D., Shen, J., Traner, A., Hensley, S. C., Lo, H.-H., Abbruzzese, J. L., & Li, D. (2006). Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF. J Biomol Tech, 17(2), 145–156.
Person, Maria D., Jianjun Shen, Angelina Traner, Sean C. Hensley, Herng-Hsiang Lo, James L. Abbruzzese, and Donghui Li. “Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF.” J Biomol Tech 17, no. 2 (April 2006): 145–56.
Person MD, Shen J, Traner A, Hensley SC, Lo H-H, Abbruzzese JL, et al. Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF. J Biomol Tech. 2006 Apr;17(2):145–56.
Person, Maria D., et al. “Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF.” J Biomol Tech, vol. 17, no. 2, Apr. 2006, pp. 145–56.
Person MD, Shen J, Traner A, Hensley SC, Lo H-H, Abbruzzese JL, Li D. Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF. J Biomol Tech. 2006 Apr;17(2):145–156.
Published In
J Biomol Tech
ISSN
1524-0215
Publication Date
April 2006
Volume
17
Issue
2
Start / End Page
145 / 156
Location
United States
Related Subject Headings
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Proteomics
- Proteins
- Protein Structure, Tertiary
- Protein Conformation
- Peptides
- Peptide Hydrolases
- Pancreas
- Molecular Sequence Data
- Molecular Chaperones