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Near-infrared optical imaging of integrin alphavbeta3 in human tumor xenografts.

Publication ,  Journal Article
Wang, W; Ke, S; Wu, Q; Charnsangavej, C; Gurfinkel, M; Gelovani, JG; Abbruzzese, JL; Sevick-Muraca, EM; Li, C
Published in: Mol Imaging
October 2004

In vivo optical imaging is potentially useful for evaluating the presence of tumor markers that are targets of molecular medicine. Here we report the synthesis and characterization of integrin alphavbeta3-targeted peptide cyclo(Lys-Arg-Gly-Asp-Phe) [c(KRGDf )] labeled with fluorescence dyes with wavelength spanning from the visible/near infrared (Cy5.5) to the true near infrared (IRDye800) for optical imaging. In vitro, the peptide-dye conjugates bound specifically to tumor cells expressing alphavbeta3. When administered intravenously into mice at a dose of 6 nmol /mouse, the conjugates accumulated in tumors expressing alphavbeta3. The tumor-to-background ratios for human KS1767 Kaposi's sarcoma in mice injected with Cy5.5-c(KRGDf ) and Cy5.5 were 5.5 and 1.5, respectively. Preinjection of c(KRGDf ) blocked the uptake of Cy5.5-c(KRGDf ) in tumors by 89%. In alphavbeta3-positive M21 and alphavbeta3-negative M21-L human melanoma, fluorescence intensity in the tumor of mice injected with IRDye800 - c(KRGDf ) was 2.3 and 1.3 times that in normal tissue, respectively. Dynamic imaging revealed that Cy5.5- c(KRGDf ) was rapidly taken up by KS1767 tumor immediately after bolus injection. The rate of its uptake in the tumor was reduced by preinjection of c(KRGDf ) in an interval time-dependent manner. Our data suggest that near-infrared fluorescence imaging may be applied to the detection of tumors expressing integrin alphavbeta3 and to the assessment of the optimal biological dose and schedule of targeted therapies.

Duke Scholars

Published In

Mol Imaging

DOI

ISSN

1535-3508

Publication Date

October 2004

Volume

3

Issue

4

Start / End Page

343 / 351

Location

England

Related Subject Headings

  • Transplantation, Heterologous
  • Sarcoma, Kaposi
  • Peptides, Cyclic
  • Nuclear Medicine & Medical Imaging
  • Neoplasms, Experimental
  • Neoplasm Transplantation
  • Microscopy, Fluorescence
  • Mice, Nude
  • Mice
  • Melanoma, Experimental
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, W., Ke, S., Wu, Q., Charnsangavej, C., Gurfinkel, M., Gelovani, J. G., … Li, C. (2004). Near-infrared optical imaging of integrin alphavbeta3 in human tumor xenografts. Mol Imaging, 3(4), 343–351. https://doi.org/10.1162/15353500200404148
Wang, Wei, Shi Ke, Qingping Wu, Chusilp Charnsangavej, Mikhail Gurfinkel, Juri G. Gelovani, James L. Abbruzzese, Eva M. Sevick-Muraca, and Chun Li. “Near-infrared optical imaging of integrin alphavbeta3 in human tumor xenografts.Mol Imaging 3, no. 4 (October 2004): 343–51. https://doi.org/10.1162/15353500200404148.
Wang W, Ke S, Wu Q, Charnsangavej C, Gurfinkel M, Gelovani JG, et al. Near-infrared optical imaging of integrin alphavbeta3 in human tumor xenografts. Mol Imaging. 2004 Oct;3(4):343–51.
Wang, Wei, et al. “Near-infrared optical imaging of integrin alphavbeta3 in human tumor xenografts.Mol Imaging, vol. 3, no. 4, Oct. 2004, pp. 343–51. Pubmed, doi:10.1162/15353500200404148.
Wang W, Ke S, Wu Q, Charnsangavej C, Gurfinkel M, Gelovani JG, Abbruzzese JL, Sevick-Muraca EM, Li C. Near-infrared optical imaging of integrin alphavbeta3 in human tumor xenografts. Mol Imaging. 2004 Oct;3(4):343–351.

Published In

Mol Imaging

DOI

ISSN

1535-3508

Publication Date

October 2004

Volume

3

Issue

4

Start / End Page

343 / 351

Location

England

Related Subject Headings

  • Transplantation, Heterologous
  • Sarcoma, Kaposi
  • Peptides, Cyclic
  • Nuclear Medicine & Medical Imaging
  • Neoplasms, Experimental
  • Neoplasm Transplantation
  • Microscopy, Fluorescence
  • Mice, Nude
  • Mice
  • Melanoma, Experimental