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Biostable electrospun microfibrous scaffolds mitigate hypertrophic scar contraction in an immune-competent murine model.

Publication ,  Journal Article
Lorden, ER; Miller, KJ; Ibrahim, MM; Bashirov, L; Hammett, E; Chakraborty, S; Quiles-Torres, C; Selim, MA; Leong, KW; Levinson, H
Published in: Acta Biomater
March 1, 2016

UNLABELLED: Burn injuries in the United States account for over one million hospital admissions per year, with treatment estimated at four billion dollars. Of severe burn patients, 30-90% will develop hypertrophic scars (HSc). In this study, we evaluate the impact of an elastomeric, randomly-oriented biostable polyurethane (PU) scaffold on HSc-related outcomes. In vitro, fibroblast-seeded PU scaffolds contracted significantly less and demonstrated fewer αSMA(+) myofibroblasts compared to fibroblast-seeded collagen lattices. In a murine HSc model, collagen coated PU (ccPU) scaffolds significantly reduced HSc contraction as compared to untreated control wounds and wounds treated with the clinical standard of care. Our data suggest that electrospun ccPU scaffolds meet the requirements to reduce HSc contraction including reduction of in vitro HSc related outcomes, diminished scar stiffness, and reduced scar contraction. While clinical dogma suggests treating severe burn patients with rapidly biodegrading skin equivalents, our data suggest that a more long-term scaffold may possess merit in reducing HSc. STATEMENT OF SIGNIFICANCE: In severe burns treated with skin grafting, between 30% and 90% of patients develop hypertrophic scars (HSc). There are no therapies to prevent HSc, and treatments are marginally effective. This work is the first example we are aware of which studies the impact of a permanent electrospun elastomer on HSc contraction in a murine model that mimics the human condition. Collagen coated polyurethane scaffolds decrease αSMA+ myofibroblast formation in vitro, prevent stiffening of scar tissue, and mitigate HSc contraction. Unlike current standards of care, electrospun, polyurethane scaffolds do not lose architecture over time. We propose that the future bioengineering strategy of mitigating HSc contraction should consider a long-term elastomeric matrix which persists within the wound bed throughout the remodeling phase of repair.

Duke Scholars

Published In

Acta Biomater

DOI

EISSN

1878-7568

Publication Date

March 1, 2016

Volume

32

Start / End Page

100 / 109

Location

England

Related Subject Headings

  • Wound Healing
  • Tissue Scaffolds
  • Tissue Engineering
  • Polyurethanes
  • Mice, Inbred C57BL
  • Immunocompetence
  • Humans
  • Fibroblasts
  • Female
  • Disease Models, Animal
 

Citation

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Lorden, E. R., Miller, K. J., Ibrahim, M. M., Bashirov, L., Hammett, E., Chakraborty, S., … Levinson, H. (2016). Biostable electrospun microfibrous scaffolds mitigate hypertrophic scar contraction in an immune-competent murine model. Acta Biomater, 32, 100–109. https://doi.org/10.1016/j.actbio.2015.12.025
Lorden, Elizabeth R., Kyle J. Miller, Mohamed M. Ibrahim, Latif Bashirov, Ellen Hammett, Syandan Chakraborty, Carlos Quiles-Torres, M Angelica Selim, Kam W. Leong, and Howard Levinson. “Biostable electrospun microfibrous scaffolds mitigate hypertrophic scar contraction in an immune-competent murine model.Acta Biomater 32 (March 1, 2016): 100–109. https://doi.org/10.1016/j.actbio.2015.12.025.
Lorden ER, Miller KJ, Ibrahim MM, Bashirov L, Hammett E, Chakraborty S, et al. Biostable electrospun microfibrous scaffolds mitigate hypertrophic scar contraction in an immune-competent murine model. Acta Biomater. 2016 Mar 1;32:100–9.
Lorden, Elizabeth R., et al. “Biostable electrospun microfibrous scaffolds mitigate hypertrophic scar contraction in an immune-competent murine model.Acta Biomater, vol. 32, Mar. 2016, pp. 100–09. Pubmed, doi:10.1016/j.actbio.2015.12.025.
Lorden ER, Miller KJ, Ibrahim MM, Bashirov L, Hammett E, Chakraborty S, Quiles-Torres C, Selim MA, Leong KW, Levinson H. Biostable electrospun microfibrous scaffolds mitigate hypertrophic scar contraction in an immune-competent murine model. Acta Biomater. 2016 Mar 1;32:100–109.
Journal cover image

Published In

Acta Biomater

DOI

EISSN

1878-7568

Publication Date

March 1, 2016

Volume

32

Start / End Page

100 / 109

Location

England

Related Subject Headings

  • Wound Healing
  • Tissue Scaffolds
  • Tissue Engineering
  • Polyurethanes
  • Mice, Inbred C57BL
  • Immunocompetence
  • Humans
  • Fibroblasts
  • Female
  • Disease Models, Animal