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Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model.

Publication ,  Journal Article
Li, F; Wiegman, C; Seiffert, JM; Zhu, J; Clarke, C; Chang, Y; Bhavsar, P; Adcock, I; Zhang, J; Zhou, X; Chung, KF
Published in: PloS one
January 2013

Chronic exposure to high levels of ozone induces emphysema and chronic inflammation in mice. We determined the recovery from ozone-induced injury and whether an antioxidant, N-acetylcysteine (NAC), could prevent or reverse the lung damage.Mice were exposed to ozone (2.5 ppm, 3 hours/12 exposures, over 6 weeks) and studied 24 hours (24h) or 6 weeks (6W) later. Nac (100 mg/kg, intraperitoneally) was administered either before each exposure (preventive) or after completion of exposure (therapeutic) for 6 weeks.After ozone exposure, there was an increase in functional residual capacity, total lung volume, and lung compliance, and a reduction in the ratio of forced expiratory volume at 25 and 50 milliseconds to forced vital capacity (FEV25/FVC, FEV50/FVC). Mean linear intercept (Lm) and airway hyperresponsiveness (AHR) to acetylcholine increased, and remained unchanged at 6W after cessation of exposure. Preventive NAC reduced the number of BAL macrophages and airway smooth muscle (ASM) mass. Therapeutic NAC reversed AHR, and reduced ASM mass and apoptotic cells.Emphysema and lung function changes were irreversible up to 6W after cessation of ozone exposure, and were not reversed by NAC. The beneficial effects of therapeutic NAC may be restricted to the ASM.

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Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2013

Volume

8

Issue

11

Start / End Page

e80782

Related Subject Headings

  • Respiratory Function Tests
  • Pulmonary Disease, Chronic Obstructive
  • Ozone
  • Mice
  • Malondialdehyde
  • Lung
  • General Science & Technology
  • Gene Expression
  • Expectorants
  • Emphysema
 

Citation

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Li, F., Wiegman, C., Seiffert, J. M., Zhu, J., Clarke, C., Chang, Y., … Chung, K. F. (2013). Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model. PloS One, 8(11), e80782. https://doi.org/10.1371/journal.pone.0080782
Li, Feng, Cornelis Wiegman, Joanna M. Seiffert, Jie Zhu, Colin Clarke, Yan Chang, Pank Bhavsar, et al. “Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model.PloS One 8, no. 11 (January 2013): e80782. https://doi.org/10.1371/journal.pone.0080782.
Li F, Wiegman C, Seiffert JM, Zhu J, Clarke C, Chang Y, et al. Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model. PloS one. 2013 Jan;8(11):e80782.
Li, Feng, et al. “Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model.PloS One, vol. 8, no. 11, Jan. 2013, p. e80782. Epmc, doi:10.1371/journal.pone.0080782.
Li F, Wiegman C, Seiffert JM, Zhu J, Clarke C, Chang Y, Bhavsar P, Adcock I, Zhang J, Zhou X, Chung KF. Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model. PloS one. 2013 Jan;8(11):e80782.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2013

Volume

8

Issue

11

Start / End Page

e80782

Related Subject Headings

  • Respiratory Function Tests
  • Pulmonary Disease, Chronic Obstructive
  • Ozone
  • Mice
  • Malondialdehyde
  • Lung
  • General Science & Technology
  • Gene Expression
  • Expectorants
  • Emphysema