Scholars@Duke publication: Human anti-pneumococcal polysaccharide antibodies are secreted by the CD5- B cell lineage.

Human anti-pneumococcal polysaccharide antibodies are secreted by the CD5- B cell lineage.

Publication , Journal Article

Barrett, DJ; Sleasman, JW; Schatz, DA; Steinitz, M

Published in: Cell Immunol

August 1992

To determine whether human antibody responses to T cell-independent pneumococcal polysaccharide antigens are derived from CD5+ or CD5- B cells, we utilized an ELISPOT assay to detect individual anti-polysaccharide antibody-secreting cells. Human anti-type IV pneumococcal polysaccharide antibody-secreting cells were found in the CD5- B cell subpopulation. An EBV transformed anti-pneumococcal antibody-secreting B cell line was also CD5-. The ontogeny of CD5 expressing B cells correlated with the age at which polysaccharide responsiveness is acquired (generally around age 2 years in humans). The CD5- B cell subset represents only 25-30% of the B cells in young children, but this fraction increases throughout childhood to a plateau of 70-80% of the B cells in adults. These results support the hypothesis that the developmental change in responsiveness to T cell-independent polysaccharide antigens in humans is associated with maturation of the CD5- B cell subset.

Duke Scholars

Author John William Sleasman Pediatrics, Allergy and Immunology

Published In

Cell Immunol

DOI

10.1016/0008-8749(92)90006-b

ISSN

0008-8749

Publication Date

August 1992

Volume

143

Issue

Start / End Page

66 / 79

Location

Netherlands

Related Subject Headings

Streptococcus pneumoniae
Polysaccharides, Bacterial
Middle Aged
Infant, Newborn
Infant
Immunology
Immunization
Humans
Child, Preschool
Child

Citation

APA

Chicago

ICMJE

MLA

NLM

Barrett, D. J., Sleasman, J. W., Schatz, D. A., & Steinitz, M. (1992). Human anti-pneumococcal polysaccharide antibodies are secreted by the CD5- B cell lineage. Cell Immunol, 143(1), 66–79. https://doi.org/10.1016/0008-8749(92)90006-b

Barrett, D. J., J. W. Sleasman, D. A. Schatz, and M. Steinitz. “Human anti-pneumococcal polysaccharide antibodies are secreted by the CD5- B cell lineage.” Cell Immunol 143, no. 1 (August 1992): 66–79. https://doi.org/10.1016/0008-8749(92)90006-b.

Barrett DJ, Sleasman JW, Schatz DA, Steinitz M. Human anti-pneumococcal polysaccharide antibodies are secreted by the CD5- B cell lineage. Cell Immunol. 1992 Aug;143(1):66–79.

Barrett, D. J., et al. “Human anti-pneumococcal polysaccharide antibodies are secreted by the CD5- B cell lineage.” Cell Immunol, vol. 143, no. 1, Aug. 1992, pp. 66–79. Pubmed, doi:10.1016/0008-8749(92)90006-b.

Barrett DJ, Sleasman JW, Schatz DA, Steinitz M. Human anti-pneumococcal polysaccharide antibodies are secreted by the CD5- B cell lineage. Cell Immunol. 1992 Aug;143(1):66–79.