Whole-mount in situ hybridization on murine skeletogenic tissues
Publication
, Journal Article
Rutkowsky, T; Sharma, D; Hilton, MJ
Published in: Methods in Molecular Biology
January 1, 2014
Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction. © 2014 Springer Science+Business Media, LLC.
Duke Scholars
Published In
Methods in Molecular Biology
DOI
ISSN
1064-3745
Publication Date
January 1, 2014
Volume
1130
Start / End Page
193 / 201
Related Subject Headings
- Developmental Biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Rutkowsky, T., Sharma, D., & Hilton, M. J. (2014). Whole-mount in situ hybridization on murine skeletogenic tissues. Methods in Molecular Biology, 1130, 193–201. https://doi.org/10.1007/978-1-62703-989-5-14
Rutkowsky, T., D. Sharma, and M. J. Hilton. “Whole-mount in situ hybridization on murine skeletogenic tissues.” Methods in Molecular Biology 1130 (January 1, 2014): 193–201. https://doi.org/10.1007/978-1-62703-989-5-14.
Rutkowsky T, Sharma D, Hilton MJ. Whole-mount in situ hybridization on murine skeletogenic tissues. Methods in Molecular Biology. 2014 Jan 1;1130:193–201.
Rutkowsky, T., et al. “Whole-mount in situ hybridization on murine skeletogenic tissues.” Methods in Molecular Biology, vol. 1130, Jan. 2014, pp. 193–201. Scopus, doi:10.1007/978-1-62703-989-5-14.
Rutkowsky T, Sharma D, Hilton MJ. Whole-mount in situ hybridization on murine skeletogenic tissues. Methods in Molecular Biology. 2014 Jan 1;1130:193–201.
Published In
Methods in Molecular Biology
DOI
ISSN
1064-3745
Publication Date
January 1, 2014
Volume
1130
Start / End Page
193 / 201
Related Subject Headings
- Developmental Biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences