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Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response.

Publication ,  Journal Article
Storozhenko, S; Navarrete, O; Ravanel, S; De Brouwer, V; Chaerle, P; Zhang, G-F; Bastien, O; Lambert, W; Rébeillé, F; Van Der Straeten, D
Published in: J Biol Chem
April 6, 2007

In plants, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase (mitHPPK/DHPS) is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis. Mining the Arabidopsis genome data base has revealed a second gene encoding a protein that lacks a potential transit peptide, suggesting a cytosolic localization of the isoenzyme (cytHPPK/DHPS). When the N-terminal part of the cytHPPK/DHPS was fused to green fluorescent protein, the fusion protein appeared only in the cytosol, confirming the above prediction. Functionality of cytHPPK/DHPS was addressed by two parallel approaches: first, the cytHPPK/DHPS was able to rescue yeast mutants lacking corresponding activities; second, recombinant cytHPPK/DHPS expressed and purified from Escherichia coli displayed both HPPK and DHPS activities in vitro. In contrast to mitHPPK/DHPS, which was ubiquitously expressed, the cytHPPK/DHPS gene was exclusively expressed in reproductive tissue, more precisely in developing seeds as revealed by histochemical analysis of a transgenic cytHPPK/DHPS promoter-GUS line. In addition, it was observed that expression of cytHPPK/DHPS mRNA was induced by salt stress, suggesting a potential role of the enzyme in stress response. This was supported by the phenotype of a T-DNA insertion mutant in the cytHPPK/DHPS gene, resulting in lower germination rates as compared with the wild-type upon application of oxidative and osmotic stress.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

April 6, 2007

Volume

282

Issue

14

Start / End Page

10749 / 10761

Location

United States

Related Subject Headings

  • Tetrahydrofolates
  • Plant Proteins
  • Oxidative Stress
  • Osmotic Pressure
  • Mutation
  • Molecular Sequence Data
  • Mitochondria
  • Germination
  • Genome, Plant
  • Diphosphotransferases
 

Citation

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Storozhenko, S., Navarrete, O., Ravanel, S., De Brouwer, V., Chaerle, P., Zhang, G.-F., … Van Der Straeten, D. (2007). Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response. J Biol Chem, 282(14), 10749–10761. https://doi.org/10.1074/jbc.M701158200
Storozhenko, Sergei, Oscar Navarrete, Stéphane Ravanel, Veerle De Brouwer, Peter Chaerle, Guo-Fang Zhang, Olivier Bastien, Willy Lambert, Fabrice Rébeillé, and Dominique Van Der Straeten. “Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response.J Biol Chem 282, no. 14 (April 6, 2007): 10749–61. https://doi.org/10.1074/jbc.M701158200.
Storozhenko S, Navarrete O, Ravanel S, De Brouwer V, Chaerle P, Zhang G-F, et al. Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response. J Biol Chem. 2007 Apr 6;282(14):10749–61.
Storozhenko, Sergei, et al. “Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response.J Biol Chem, vol. 282, no. 14, Apr. 2007, pp. 10749–61. Pubmed, doi:10.1074/jbc.M701158200.
Storozhenko S, Navarrete O, Ravanel S, De Brouwer V, Chaerle P, Zhang G-F, Bastien O, Lambert W, Rébeillé F, Van Der Straeten D. Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response. J Biol Chem. 2007 Apr 6;282(14):10749–10761.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

April 6, 2007

Volume

282

Issue

14

Start / End Page

10749 / 10761

Location

United States

Related Subject Headings

  • Tetrahydrofolates
  • Plant Proteins
  • Oxidative Stress
  • Osmotic Pressure
  • Mutation
  • Molecular Sequence Data
  • Mitochondria
  • Germination
  • Genome, Plant
  • Diphosphotransferases