Skip to main content

Investigation of the extraction behavior of the main monoglutamate folates from spinach by liquid chromatography-electrospray ionization tandem mass spectrometry.

Publication ,  Journal Article
Zhang, G-F; Storozhenko, S; Van Der Straeten, D; Lambert, WE
Published in: J Chromatogr A
June 17, 2005

An LC-MS/MS method has been developed for the determination of main monoglutamate folates in spinach with folic acid as an internal standard. A sample preparation with ultrafiltration (molecular weight cut-off membrane, 5 kDa) was followed by a chromatographic run of 14.2 min, rendering the method very simple and fast. The LODs in diluted spinach matrix were 0.02, 0.09, 0.05 and 0.03 ng/mL (0.037, 0.17, 0.092 and 0.055 microg/100 g calculated according to the fresh weight of spinach) for 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolate, and 10-formylfolic acid, respectively. Using this method, the extraction behaviour of the main naturally occurring monoglutamate folates has been investigated in detail. It is found that 10 min of heating at 100 degrees C, incubation with rat serum at 37 degrees C (0.05 M phosphate buffer, pH = 6.5) for 4 h and the ratio of 10 (volume of extraction buffer versus the weight of sample, mL/g) are the optimal parameters for folate extraction from spinach. The final quantitative result of the individual folates in spinach is highly influenced by the pH (from 2.9 to 8.6) of the extraction buffer.

Duke Scholars

Published In

J Chromatogr A

DOI

ISSN

0021-9673

Publication Date

June 17, 2005

Volume

1078

Issue

1-2

Start / End Page

59 / 66

Location

Netherlands

Related Subject Headings

  • gamma-Glutamyl Hydrolase
  • Time Factors
  • Spinacia oleracea
  • Spectrometry, Mass, Electrospray Ionization
  • Hydrogen-Ion Concentration
  • Hot Temperature
  • Folic Acid
  • Drug Stability
  • Chromatography, High Pressure Liquid
  • Chemical Fractionation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, G.-F., Storozhenko, S., Van Der Straeten, D., & Lambert, W. E. (2005). Investigation of the extraction behavior of the main monoglutamate folates from spinach by liquid chromatography-electrospray ionization tandem mass spectrometry. J Chromatogr A, 1078(1–2), 59–66. https://doi.org/10.1016/j.chroma.2005.04.085
Zhang, Guo-Fang, Sergei Storozhenko, Dominique Van Der Straeten, and Willy E. Lambert. “Investigation of the extraction behavior of the main monoglutamate folates from spinach by liquid chromatography-electrospray ionization tandem mass spectrometry.J Chromatogr A 1078, no. 1–2 (June 17, 2005): 59–66. https://doi.org/10.1016/j.chroma.2005.04.085.
Zhang, Guo-Fang, et al. “Investigation of the extraction behavior of the main monoglutamate folates from spinach by liquid chromatography-electrospray ionization tandem mass spectrometry.J Chromatogr A, vol. 1078, no. 1–2, June 2005, pp. 59–66. Pubmed, doi:10.1016/j.chroma.2005.04.085.

Published In

J Chromatogr A

DOI

ISSN

0021-9673

Publication Date

June 17, 2005

Volume

1078

Issue

1-2

Start / End Page

59 / 66

Location

Netherlands

Related Subject Headings

  • gamma-Glutamyl Hydrolase
  • Time Factors
  • Spinacia oleracea
  • Spectrometry, Mass, Electrospray Ionization
  • Hydrogen-Ion Concentration
  • Hot Temperature
  • Folic Acid
  • Drug Stability
  • Chromatography, High Pressure Liquid
  • Chemical Fractionation