Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Ultrasound-mediated release of hydrophilic and lipophilic agents from echogenic liposomes.

Publication ,  Journal Article
Kopechek, JA; Abruzzo, TM; Wang, B; Chrzanowski, SM; Smith, DAB; Kee, PH; Huang, S; Collier, JH; McPherson, DD; Holland, CK
Published in: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
November 2008

To achieve ultrasound-controlled drug delivery using echogenic liposomes (ELIPs), we assessed ultrasound-triggered release of hydrophilic and lipophilic agents in vitro using color Doppler ultrasound delivered with a clinical 6-MHz compact linear array transducer.Calcein, a hydrophilic agent, and papaverine, a lipophilic agent, were each separately loaded into ELIPs. Calcein-loaded ELIP (C-ELIP) and papaverine-loaded ELIP (P-ELIP) solutions were circulated in a flow model and treated with 6-MHz color Doppler ultrasound or Triton X-100. Treatment with Triton X-100 was used to release the encapsulated calcein or papaverine content completely. The free calcein concentration in the solution was measured directly by spectrofluorimetry. The free papaverine in the solution was separated from liposome-bound papaverine by spin column filtration, and the resulting papaverine concentration was measured directly by absorbance spectrophotometry. Dynamic changes in echogenicity were assessed with low-output B-mode ultrasound (mechanical index, 0.04) as mean digital intensity.Color Doppler ultrasound caused calcein release from C-ELIPs compared with flow alone (P < .05) but did not induce papaverine release from P-ELIPs compared with flow alone (P > .05). Triton X-100 completely released liposome-associated calcein and papaverine. Initial echogenicity was higher for C-ELIPs than P-ELIPs. Color Doppler ultrasound and Triton X-100 treatments reduced echogenicity for both C-ELIPs and P-ELIPs (P < .05).The differential efficiency of ultrasound-mediated pharmaceutical release from ELIPs for water- and lipid-soluble compounds suggests that water-soluble drugs are better candidates for the design and development of ELIP-based ultrasound-controlled drug delivery systems.

Duke Scholars

Published In

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

DOI

EISSN

1550-9613

ISSN

0278-4297

Publication Date

November 2008

Volume

27

Issue

11

Start / End Page

1597 / 1606

Related Subject Headings

  • Sonication
  • Phosphodiesterase Inhibitors
  • Papaverine
  • Nuclear Medicine & Medical Imaging
  • Liposomes
  • Hydrophobic and Hydrophilic Interactions
  • Fluoresceins
  • Drug Delivery Systems
  • Diffusion
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kopechek, J. A., Abruzzo, T. M., Wang, B., Chrzanowski, S. M., Smith, D. A. B., Kee, P. H., … Holland, C. K. (2008). Ultrasound-mediated release of hydrophilic and lipophilic agents from echogenic liposomes. Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine, 27(11), 1597–1606. https://doi.org/10.7863/jum.2008.27.11.1597
Kopechek, Jonathan A., Todd M. Abruzzo, Boyu Wang, Stephen M. Chrzanowski, Denise A. B. Smith, Patrick H. Kee, Shaoling Huang, Joel H. Collier, David D. McPherson, and Christy K. Holland. “Ultrasound-mediated release of hydrophilic and lipophilic agents from echogenic liposomes.Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine 27, no. 11 (November 2008): 1597–1606. https://doi.org/10.7863/jum.2008.27.11.1597.
Kopechek JA, Abruzzo TM, Wang B, Chrzanowski SM, Smith DAB, Kee PH, et al. Ultrasound-mediated release of hydrophilic and lipophilic agents from echogenic liposomes. Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine. 2008 Nov;27(11):1597–606.
Kopechek, Jonathan A., et al. “Ultrasound-mediated release of hydrophilic and lipophilic agents from echogenic liposomes.Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine, vol. 27, no. 11, Nov. 2008, pp. 1597–606. Epmc, doi:10.7863/jum.2008.27.11.1597.
Kopechek JA, Abruzzo TM, Wang B, Chrzanowski SM, Smith DAB, Kee PH, Huang S, Collier JH, McPherson DD, Holland CK. Ultrasound-mediated release of hydrophilic and lipophilic agents from echogenic liposomes. Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine. 2008 Nov;27(11):1597–1606.

Published In

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

DOI

EISSN

1550-9613

ISSN

0278-4297

Publication Date

November 2008

Volume

27

Issue

11

Start / End Page

1597 / 1606

Related Subject Headings

  • Sonication
  • Phosphodiesterase Inhibitors
  • Papaverine
  • Nuclear Medicine & Medical Imaging
  • Liposomes
  • Hydrophobic and Hydrophilic Interactions
  • Fluoresceins
  • Drug Delivery Systems
  • Diffusion
  • 3202 Clinical sciences