Coupling spatial segregation with synthetic circuits to control bacterial survival.
Engineered bacteria have great potential for medical and environmental applications. Fulfilling this potential requires controllability over engineered behaviors and scalability of the engineered systems. Here, we present a platform technology, microbial swarmbot, which employs spatial arrangement to control the growth dynamics of engineered bacteria. As a proof of principle, we demonstrated a safeguard strategy to prevent unintended bacterial proliferation. In particular, we adopted several synthetic gene circuits to program collective survival in Escherichia coli: the engineered bacteria could only survive when present at sufficiently high population densities. When encapsulated by permeable membranes, these bacteria can sense the local environment and respond accordingly. The cells inside the microbial swarmbot capsules will survive due to their high densities. Those escaping from a capsule, however, will be killed due to a decrease in their densities. We demonstrate that this design concept is modular and readily generalizable. Our work lays the foundation for engineering integrated and programmable control of hybrid biological-material systems for diverse applications.
Duke Scholars
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Related Subject Headings
- Systems Biology
- Polylysine
- Models, Molecular
- Microbial Viability
- Lab-On-A-Chip Devices
- Genetic Engineering
- Gene Regulatory Networks
- Gene Expression Regulation, Bacterial
- Escherichia coli
- Bioinformatics
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Systems Biology
- Polylysine
- Models, Molecular
- Microbial Viability
- Lab-On-A-Chip Devices
- Genetic Engineering
- Gene Regulatory Networks
- Gene Expression Regulation, Bacterial
- Escherichia coli
- Bioinformatics