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How the kinetochore couples microtubule force and centromere stretch to move chromosomes.

Publication ,  Journal Article
Suzuki, A; Badger, BL; Haase, J; Ohashi, T; Erickson, HP; Salmon, ED; Bloom, K
Published in: Nat Cell Biol
April 2016

The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization and depolymerization. Spc24/Spc25 at one end of the Ndc80 complex binds the kinetochore. The N-terminal tail and CH domains of Ndc80 bind microtubules, and an internal domain binds microtubule-associated proteins (MAPs) such as the Dam1 complex. To determine how the microtubule- and MAP-binding domains of Ndc80 contribute to force production at the kinetochore in budding yeast, we have inserted a FRET tension sensor into the Ndc80 protein about halfway between its microtubule-binding and internal loop domains. The data support a mechanical model of force generation at metaphase where the position of the kinetochore relative to the microtubule plus end reflects the relative strengths of microtubule depolymerization, centromere stretch and microtubule-binding interactions with the Ndc80 and Dam1 complexes.

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Published In

Nat Cell Biol

DOI

EISSN

1476-4679

Publication Date

April 2016

Volume

18

Issue

4

Start / End Page

382 / 392

Location

England

Related Subject Headings

  • Time-Lapse Imaging
  • Saccharomycetales
  • Saccharomyces cerevisiae Proteins
  • Protein Binding
  • Nuclear Proteins
  • Mutation
  • Models, Biological
  • Microtubules
  • Microtubule-Associated Proteins
  • Microscopy, Fluorescence
 

Citation

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Suzuki, A., Badger, B. L., Haase, J., Ohashi, T., Erickson, H. P., Salmon, E. D., & Bloom, K. (2016). How the kinetochore couples microtubule force and centromere stretch to move chromosomes. Nat Cell Biol, 18(4), 382–392. https://doi.org/10.1038/ncb3323
Suzuki, Aussie, Benjamin L. Badger, Julian Haase, Tomoo Ohashi, Harold P. Erickson, Edward D. Salmon, and Kerry Bloom. “How the kinetochore couples microtubule force and centromere stretch to move chromosomes.Nat Cell Biol 18, no. 4 (April 2016): 382–92. https://doi.org/10.1038/ncb3323.
Suzuki A, Badger BL, Haase J, Ohashi T, Erickson HP, Salmon ED, et al. How the kinetochore couples microtubule force and centromere stretch to move chromosomes. Nat Cell Biol. 2016 Apr;18(4):382–92.
Suzuki, Aussie, et al. “How the kinetochore couples microtubule force and centromere stretch to move chromosomes.Nat Cell Biol, vol. 18, no. 4, Apr. 2016, pp. 382–92. Pubmed, doi:10.1038/ncb3323.
Suzuki A, Badger BL, Haase J, Ohashi T, Erickson HP, Salmon ED, Bloom K. How the kinetochore couples microtubule force and centromere stretch to move chromosomes. Nat Cell Biol. 2016 Apr;18(4):382–392.

Published In

Nat Cell Biol

DOI

EISSN

1476-4679

Publication Date

April 2016

Volume

18

Issue

4

Start / End Page

382 / 392

Location

England

Related Subject Headings

  • Time-Lapse Imaging
  • Saccharomycetales
  • Saccharomyces cerevisiae Proteins
  • Protein Binding
  • Nuclear Proteins
  • Mutation
  • Models, Biological
  • Microtubules
  • Microtubule-Associated Proteins
  • Microscopy, Fluorescence