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Deletion of p47phox attenuates angiotensin II-induced abdominal aortic aneurysm formation in apolipoprotein E-deficient mice.

Publication ,  Journal Article
Thomas, M; Gavrila, D; McCormick, ML; Miller, FJ; Daugherty, A; Cassis, LA; Dellsperger, KC; Weintraub, NL
Published in: Circulation
August 1, 2006

BACKGROUND: Angiotensin II (Ang II) contributes to vascular pathology in part by stimulating NADPH oxidase activity, leading to increased formation of superoxide (O2-). We reported that O2- levels, NADPH oxidase activity, and expression of the p47phox subunit of NADPH oxidase are increased in human abdominal aortic aneurysms (AAAs). Here, we tested the hypothesis that deletion of p47phox will attenuate oxidative stress and AAA formation in Ang II-infused apoE-/- mice. METHODS AND RESULTS: Male apoE-/- and apoE-/-p47phox-/- mice received saline or Ang II (1000 ng x kg(-1) x min(-1)) infusion for 28 days, after which abdominal aortic weight and maximal diameter were determined. Aortic tissues and blood were examined for parameters of aneurysmal disease and oxidative stress. Ang II infusion induced AAAs in 90% of apoE-/- versus 16% of apo-/-p47phox-/- mice (P < 0.05). Abdominal aortic weight (14.1 +/- 3.2 versus 35.6 +/- 9.0 mg), maximal aortic diameter (1.5 +/- 0.2 versus 2.4 +/- 0.4 mm), aortic NADPH oxidase activity, and parameters of oxidative stress were reduced in apoE-/-p47phox-/- mice compared with apoE-/- mice (P < 0.05). In addition, aortic macrophage infiltration and matrix metalloproteinase-2 activity were reduced in apoE-/-p47phox-/- mice compared with apoE-/- mice. Deletion of p47phox attenuated the pressor response to Ang II; however, coinfusion of phenylephrine with Ang II, which restored the Ang II pressor response, did not alter the protective effects of p47phox deletion on AAA formation. CONCLUSIONS: Deletion of p47phox attenuates Ang II-induced AAA formation in apoE-/- mice, suggesting that NADPH oxidase plays a critical role in AAA formation in this model.

Duke Scholars

Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

August 1, 2006

Volume

114

Issue

5

Start / End Page

404 / 413

Location

United States

Related Subject Headings

  • Triglycerides
  • Superoxides
  • Phenylephrine
  • Oxidative Stress
  • NADPH Oxidases
  • Mice, Knockout
  • Mice
  • Matrix Metalloproteinase 2
  • Male
  • Macrophages
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Thomas, M., Gavrila, D., McCormick, M. L., Miller, F. J., Daugherty, A., Cassis, L. A., … Weintraub, N. L. (2006). Deletion of p47phox attenuates angiotensin II-induced abdominal aortic aneurysm formation in apolipoprotein E-deficient mice. Circulation, 114(5), 404–413. https://doi.org/10.1161/CIRCULATIONAHA.105.607168
Thomas, Manesh, Dan Gavrila, Michael L. McCormick, Francis J. Miller, Alan Daugherty, Lisa A. Cassis, Kevin C. Dellsperger, and Neal L. Weintraub. “Deletion of p47phox attenuates angiotensin II-induced abdominal aortic aneurysm formation in apolipoprotein E-deficient mice.Circulation 114, no. 5 (August 1, 2006): 404–13. https://doi.org/10.1161/CIRCULATIONAHA.105.607168.
Thomas M, Gavrila D, McCormick ML, Miller FJ, Daugherty A, Cassis LA, et al. Deletion of p47phox attenuates angiotensin II-induced abdominal aortic aneurysm formation in apolipoprotein E-deficient mice. Circulation. 2006 Aug 1;114(5):404–13.
Thomas, Manesh, et al. “Deletion of p47phox attenuates angiotensin II-induced abdominal aortic aneurysm formation in apolipoprotein E-deficient mice.Circulation, vol. 114, no. 5, Aug. 2006, pp. 404–13. Pubmed, doi:10.1161/CIRCULATIONAHA.105.607168.
Thomas M, Gavrila D, McCormick ML, Miller FJ, Daugherty A, Cassis LA, Dellsperger KC, Weintraub NL. Deletion of p47phox attenuates angiotensin II-induced abdominal aortic aneurysm formation in apolipoprotein E-deficient mice. Circulation. 2006 Aug 1;114(5):404–413.

Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

August 1, 2006

Volume

114

Issue

5

Start / End Page

404 / 413

Location

United States

Related Subject Headings

  • Triglycerides
  • Superoxides
  • Phenylephrine
  • Oxidative Stress
  • NADPH Oxidases
  • Mice, Knockout
  • Mice
  • Matrix Metalloproteinase 2
  • Male
  • Macrophages