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Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen.

Publication ,  Journal Article
Bastidas, RJ; Valdivia, RH
Published in: Microbiol Mol Biol Rev
June 2016

Chlamydia species infect millions of individuals worldwide and are important etiological agents of sexually transmitted disease, infertility, and blinding trachoma. Historically, the genetic intractability of this intracellular pathogen has hindered the molecular dissection of virulence factors contributing to its pathogenesis. The obligate intracellular life cycle of Chlamydia and restrictions on the use of antibiotics as selectable markers have impeded the development of molecular tools to genetically manipulate these pathogens. However, recent developments in the field have resulted in significant gains in our ability to alter the genome of Chlamydia, which will expedite the elucidation of virulence mechanisms. In this review, we discuss the challenges affecting the development of molecular genetic tools for Chlamydia and the work that laid the foundation for recent advancements in the genetic analysis of this recalcitrant pathogen.

Duke Scholars

Published In

Microbiol Mol Biol Rev

DOI

EISSN

1098-5557

Publication Date

June 2016

Volume

80

Issue

2

Start / End Page

411 / 427

Location

United States

Related Subject Headings

  • Microbiology
  • Humans
  • Host-Pathogen Interactions
  • Genetic Engineering
  • Genes, Bacterial
  • Gene Transfer, Horizontal
  • Drug Resistance, Bacterial
  • DNA, Bacterial
  • Chlamydia Infections
  • Chlamydia
 

Citation

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Bastidas, R. J., & Valdivia, R. H. (2016). Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen. Microbiol Mol Biol Rev, 80(2), 411–427. https://doi.org/10.1128/MMBR.00071-15
Bastidas, Robert J., and Raphael H. Valdivia. “Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen.Microbiol Mol Biol Rev 80, no. 2 (June 2016): 411–27. https://doi.org/10.1128/MMBR.00071-15.
Bastidas RJ, Valdivia RH. Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen. Microbiol Mol Biol Rev. 2016 Jun;80(2):411–27.
Bastidas, Robert J., and Raphael H. Valdivia. “Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen.Microbiol Mol Biol Rev, vol. 80, no. 2, June 2016, pp. 411–27. Pubmed, doi:10.1128/MMBR.00071-15.
Bastidas RJ, Valdivia RH. Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen. Microbiol Mol Biol Rev. 2016 Jun;80(2):411–427.

Published In

Microbiol Mol Biol Rev

DOI

EISSN

1098-5557

Publication Date

June 2016

Volume

80

Issue

2

Start / End Page

411 / 427

Location

United States

Related Subject Headings

  • Microbiology
  • Humans
  • Host-Pathogen Interactions
  • Genetic Engineering
  • Genes, Bacterial
  • Gene Transfer, Horizontal
  • Drug Resistance, Bacterial
  • DNA, Bacterial
  • Chlamydia Infections
  • Chlamydia