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The role of monogamous bivalency and Fc interactions in the binding of anti-DNA antibodies to DNA antigen.

Publication ,  Journal Article
Stearns, NA; Pisetsky, DS
Published in: Clin Immunol
May 2016

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus. These antibodies can bind DNA avidly by monogamous bivalency, a mechanism which requires the interaction of both Fab combining regions with antigenic determinants on the same polynucleotide. To explore further this mechanism, we tested Fab and F(ab')2 fragments prepared from IgG from patient plasmas in an ELISA with native DNA antigen, detecting antibody with a peroxidase conjugated anti-Fab reagent. These studies showed that Fab fragments, which can only bind monovalently, had negligible activity. Although bivalent F(ab')2 fragments would be predicted to bind DNA, these fragments also showed poor anti-DNA activity. Control studies showed that the fragments retained antibody activity to tetanus toxoid and an EBV antigen preparation. Together, these findings suggest that anti-DNA avidity depends on monogamous bivalency, with the antibody Fc portion also influencing DNA binding, in a mechanism which can be termed Fc-dependent monogamous bivalency.

Duke Scholars

Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

May 2016

Volume

166-167

Start / End Page

38 / 47

Location

United States

Related Subject Headings

  • Tetanus Toxoid
  • Protein Binding
  • Immunology
  • Immunoglobulin G
  • Immunoglobulin Fc Fragments
  • Immunoglobulin Fab Fragments
  • Humans
  • Herpesvirus 4, Human
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
 

Citation

APA
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ICMJE
MLA
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Stearns, N. A., & Pisetsky, D. S. (2016). The role of monogamous bivalency and Fc interactions in the binding of anti-DNA antibodies to DNA antigen. Clin Immunol, 166167, 38–47. https://doi.org/10.1016/j.clim.2016.04.004
Stearns, Nancy A., and David S. Pisetsky. “The role of monogamous bivalency and Fc interactions in the binding of anti-DNA antibodies to DNA antigen.Clin Immunol 166–167 (May 2016): 38–47. https://doi.org/10.1016/j.clim.2016.04.004.
Stearns, Nancy A., and David S. Pisetsky. “The role of monogamous bivalency and Fc interactions in the binding of anti-DNA antibodies to DNA antigen.Clin Immunol, vol. 166–167, May 2016, pp. 38–47. Pubmed, doi:10.1016/j.clim.2016.04.004.
Journal cover image

Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

May 2016

Volume

166-167

Start / End Page

38 / 47

Location

United States

Related Subject Headings

  • Tetanus Toxoid
  • Protein Binding
  • Immunology
  • Immunoglobulin G
  • Immunoglobulin Fc Fragments
  • Immunoglobulin Fab Fragments
  • Humans
  • Herpesvirus 4, Human
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay