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Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy.

Publication ,  Journal Article
Devi, GR; Nath, S
Published in: Methods Mol Biol
2016

Dendritic cell (DC)-based vaccines are commonly used for cancer immunotherapy. To prepare vaccines, DCs are pulsed or transfected with either: (a) defined peptides of tumor-associated antigens, (b) total protein isolated from the tumor cell, (c) autologous total RNA isolated from the tumor cell, (d) synthetic tumor-antigen-encoding mRNA, or (e) genes that encode for specific tumor-associated antigens. Introduction of tumor-associated antigen(s) and subsequent generation of mature DCs that can stimulate tumor-antigen-specific cytotoxic T lymphocytes comprise the critical steps of cancer vaccine preparation. Here, we described a method of: (a) preparing and delivering synthetic FOXP3 mRNA into human DCs, (b) generating mature DCs,

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Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2016

Volume

1428

Start / End Page

231 / 243

Location

United States

Related Subject Headings

  • Transfection
  • T-Lymphocytes, Cytotoxic
  • RNA, Messenger
  • Inflammatory Breast Neoplasms
  • Immunotherapy
  • Humans
  • Forkhead Transcription Factors
  • Female
  • Electroporation
  • Developmental Biology
 

Citation

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Devi, G. R., & Nath, S. (2016). Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy. Methods Mol Biol, 1428, 231–243. https://doi.org/10.1007/978-1-4939-3625-0_15
Devi, Gayathri R., and Sritama Nath. “Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy.Methods Mol Biol 1428 (2016): 231–43. https://doi.org/10.1007/978-1-4939-3625-0_15.
Devi, Gayathri R., and Sritama Nath. “Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy.Methods Mol Biol, vol. 1428, 2016, pp. 231–43. Pubmed, doi:10.1007/978-1-4939-3625-0_15.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2016

Volume

1428

Start / End Page

231 / 243

Location

United States

Related Subject Headings

  • Transfection
  • T-Lymphocytes, Cytotoxic
  • RNA, Messenger
  • Inflammatory Breast Neoplasms
  • Immunotherapy
  • Humans
  • Forkhead Transcription Factors
  • Female
  • Electroporation
  • Developmental Biology